RBRC01361, B6.Cg-Trp53<tm1Sia>/Rbrc

RBRC No. RBRC01361
Type Targeted MutationCartagena
Species Mus musculus
Strain name B6.Cg-Trp53<tm1Sia>/Rbrc
Former Common name C57BL-p53+/-
H-2 Haplotype No Data
ES cell line TT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1]
Donor strain (C57BL/6 x CBA)F1 via TT2 ES cell line
Background strain C57BL/6NCrSlc
1 Appearance black
Genotype a/a B/B C/C
Strain development Developed by Shinichi Aizawa, RIKEN Tsukuba Institute. The TT2 ES cells derived from B6CBAF1 were used to disrupt the p53 gene by homologous recombination. The mutant mice were backcrossed to C57BL/6, ICR and C3H, respectively by Ohtura Niwa, Radiation Biology Center, Kyoto University, to DBA/2 by Takeshi Yagi, Graduate School of Frontier Biosciences, Osaka University, to MSM by Ryo Kominami, Niigata University Graduate School of Medical and Dental Sciences.
Strain description Trp53 gene knockout mice. A neomycin selection cassette was inserted into the second exon of the Trp53 gene. Homozygous deficient mice can grow after birth, but often suffered from development of tumors in various region of the body. Several congenic strains were generated. C57BL/6 background (RBRC01361), ICR background (RBRC01348), C3H background (RBRC00107), DBA/2 background (RBRC01518), and MSM background (RBRC00815). In C57BL/6 background most of the female homozygotes die in utero (probably, also in MSM background).
Colony maintenance Heterozygote x Wild-type [or Crossing to C57BL/6NCrSlc]
Health Report
Gene Details
Promoter No Data
1 Symbol Trp53
Symbol name transformation-related protein 53
Chromosome 11
Common name p53
Symbol description No Data
Promoter herpes simplex virus thymidine kinase promoter (HSV tk promoter)
2 Symbol neo
Symbol name neomycin resistance gene (E. coli)
Chromosome 11
Common name neo; neomycin;
Symbol description No Data
References Oncogene. 1993 Dec;8(12):3313-22.

Enhanced proliferative potential in culture of cells from p53-deficient mice.
Research applications Cancer Research,
Cell Biology Research,
Immunology and Inflammation Research,
Mouse Models for Human Disease
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
Oncogene, 8, 3313-3322 (1993).
Please inform Laboratory for Animal Resources and Genetic Engineering,
CDB (e mail: mutant.bdr@riken.jp) about the paper when published.
Additional information
1 Mouse of the Month Jun 2005
Genetic Background
Breeding characters
2 Genotyping protocol <PCR>
Depositor Niwa, Ohtsura (Radiation Biology Center, Kyoto University) Niwa, Ohtsura
Strain Status /
(Expected delivery)

Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months

Live mouse :
BRC mice in Publications
Title Journal
Etoposide induces TRP53-dependent apoptosis and TRP53-independent cell cycle arrest in trophoblasts of the developing mouse placenta. Biol Reprod.80(4): 813-22 (2009).(19109225)
Yamauchi H, Katayama K, Ueno M, Kanemitsu H, Nam C, Mikami T, Saito A, Ishida Y, Uetsuka K, Doi K, Ohmach Y, Nakayama H.
6-mercaptopurine (6-MP) induces p53-mediated apoptosis of neural progenitor cells in the developing fetal rodent brain. Neurotoxicol Teratol.31(4): 198-202 (2009).(19281843)
Kanemitsu H, Yamauchi H, Komatsu M, Yamamoto S, Okazaki S, Uchida K, Nakayama H.
Sulfation of colonic mucins by N-acetylglucosamine 6-O-sulfotransferase-2 and its protective function in experimental colitis in mice. J Biol Chem.285(9): 6750-60 (2010).(20018871)
Tobisawa Y, Imai Y, Fukuda M, Kawashima H.
Etoposide induces G2/M arrest and apoptosis in neural progenitor cells via DNA damage and an ATM/p53-related pathway. Histol Histopathol.25(4): 485-93 (2010).(20183801)
Nam C, Doi K, Nakayama H.
Roles of GlcNAc-6-O-sulfotransferases in lymphoid and nonlymphoid tissues. Methods Enzymol.479: 243-56 (2010).(20816170)
Kawashima H.
5-azacytidine, a chemotherapeutic drug, induces TRAIL-mediated apoptosis in mouse thymocytes in vivo. Exp Toxicol Pathol. (2011).(20092982)
Tochitani T, Kanemitsu H, Yamauchi H, Uchida K, Nakayama H.
Inhibitory effect of p53 on mitochondrial content and function during adipogenesis. Biochem Biophys Res Commun. (2014).(24565844)
Okita N, Ishikawa N, Mizunoe Y, Oku M, Nagai W, Suzuki Y, Matsushima S, Mikami K, Okado H, Sasaki T, Higami Y.
Undifferentiated State Induced by Rb-P53 Double Inactivation in Mouse Thyroid Neuroendocrine Cells and Embryonic Fibroblasts Stem Cells (2015).(25694388)
Kitajima S1, Kohno S, Kondoh A, Sasaki N, Nishimoto Y, Li F, Mohammed MS, Muranaka H, Nagatani N, Suzuki M, Kido Y, Takahashi C
Metabolic characterization of cultured mammalian cells by mass balance analysis, tracer labeling experiments and computer-aided simulations. J. Biosci. Bioeng. (2015).(25936961)
Okahashi N, Kohno S, Kitajima S, Matsuda F, Takahashi C, Shimizu H.
Ganglioside GD3 enhances invasiveness of gliomas by forming a complex with platelet-derived growth factor receptor alpha and Yes. J. Biol. Chem. (2015).(25940087)
Ohkawa Y, Momota H, Kato A, Hashimoto N, Tsuda Y, Kotani N, Honke K, Suzumura A, Furukawa K, Ohmi Y, Natsume A, Wakabayashi T, Furukawa K.
N-Methyl, N-propynyl-2-phenylethylamine (MPPE), a Selegiline Analog, Attenuates MPTP-induced Dopaminergic Toxicity with Guaranteed Behavioral Safety: Involvement of Inhibitions of Mitochondrial Oxidative Burdens and p53 Gene-elicited Pro-apoptotic Change. Mol. Neurobiol. (2015).(26563498)
Shin EJ, Nam Y, Lee JW, Nguyen PT, Yoo JE, Tran TV, Jeong JH, Jang CG, Oh YJ, Youdim MB, Lee PH, Nabeshima T, Kim HC.
The Transcriptional Landscape of p53 Signalling Pathway. EBioMedicine20: 109-119 (2017).(28558959)
Tanikawa C, Zhang YZ, Yamamoto R, Tsuda Y, Tanaka M, Funauchi Y, Mori J, Imoto S, Yamaguchi R, Nakamura Y, Miyano S, Nakagawa H, Matsuda K.
Development of a functional thyroid model based on an organoid culture system. Biochem. Biophys. Res. Commun. (2018).(29470983)
Saito Y, Onishi N, Takami H, Seishima R, Inoue H, Hirata Y, Kameyama K, Tsuchihashi K, Sugihara E, Uchino S, Ito K, Kawakubo H, Takeuchi H, Kitagawa Y, Saya H, Nagano O.
Identification of WWP1 as an obesity-associated E3 ubiquitin ligase with a protective role against oxidative stress in adipocytes. Biochem. Biophys. Res. Commun. (2018).(30471861)
Kobayashi M, Hoshino S, Abe T, Okita N, Tagawa R, Nagai W, Konno R, Suzuki Y, Furuya K, Ishikawa N, Okado H, Oku M, Iwamoto M, Miura Y, Sudo Y, Higami Y.
Genetic depletion of p53 attenuates cocaine-induced hepatotoxicity in mice. Biochimie. (2018).(30576773)
Mai HN, Sharma G, Sharma N, Shin EJ, Kim DJ, Pham DT, Trinh QD, Jang CG, Nah SY, Jeong JH, Kim HC.
Establishment of renal proximal tubule cell lines derived from the kidney of p53 knockout mice. Cytotechnology (2019).(30603921)
Sasaki H, Sugiyama M, Sasaki N.