Strain Data Sheet

RBRC01362

Strain Information

Image
BRC No.RBRC01362
TypeTransgeneCartagena
SpeciesMus musculus
Strain nameICR.Cg-Tg(CAG-cat,-Mesp1)1Ysa/YsaRbrc
Former Common nameCAG-CAT-Mesp1
H-2 Haplotype
ES Cell line
Background strainMCH
Appearance
Strain developmentDeveloped by Dr. Yumiko Saga, National Institute of Genetics in 2001. ICR background.
Strain descriptionThe transgene contains CAG promoter and Mesp1 cDNA separated by a floxed chloramphenicol acetyltransferase (CAT) cassette. When the floxed CAT cassette is excised by crossing with Cre mice, Mesp1 is expressed.
Colony maintenance
References

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Mesp1mesoderm posterior 1UNMesp1

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
catE. coli chloramphenicol acetyltransferase (CAT)UNCAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA)

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxPUNloxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxPUNloxP

Ordering Information

Donor DNACAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA), Phage P1 loxP sites, E. coli chloramphenicol acetyltransferase (CAT) gene, Mesp1 cDNA
Research applicationCre/loxP system
Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. Within a period of 2 years after deposition of the material, user must contact the DEPOSITOR and notify him a content of the research. If the DEPOSITOR judges that the research should be a collaborative work, then user must include DEPOSITOR and/or developer's names as co-authors.
DepositorYumiko Saga (National Institute of Genetics)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Approval form (Japanese / English)
  2. Order form (Japanese / English)
  3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  4. CAGGS MTA (English)
  5. Acceptance of responsibility for living modified organism (Japanese / English)
Saga Lab HP
Genotyping protocol -PCR-

BRC mice in Publications

No Data