RBRC01458, B6.129-Dpt<tm1Hsh>/NakaRbrc

RBRC No. RBRC01458
Type Targeted MutationCartagena
Species Mus musculus
Strain name B6.129-Dpt<tm1Hsh>/NakaRbrc
Former Common name Dermatopontin KO mice
H-2 Haplotype No Data
Donor strain (129X1/SvJ x 129S1/Sv)F1-Kitl<+>
Background strain C57BL/6JJcl
Appearance
1 Appearance black
Genotype a/a B/B C/C
Strain development Developed by Dr. Hroshi Shinkai, Chiba University at 2002. A pgk-neo was transfered into ES cells to relpace exon 1 of Dpt gene.
Strain description Dermatopontin (Dpt) is a collagen-associated protein, is widely distributed in extracellular matrix. Homozygote mutant mice not exhibit any obvious anatomical abnormality, but the skin elasticity was increased. This strain is useful for study of the mechanism and the process of collagen fibrillogenesis.
Colony maintenance Backcross to C57BL/6 (Heterozygote x C57BL/6JJcl)
Homozygote mutant mice are fertile.
Health Report No Data
Gene Details
Promoter No Data
1 Symbol Dpt
Symbol name Dermatopontin
Chromosome 1
Common name 1810032B19Rik, 5033416F05Rik, early quiescence protein-1, EQ-1
Symbol description No Data
Promoter herpes simplex virus thymidine kinase promoter (HSV tk promoter)
2 Symbol neo
Symbol name neomycin resistance gene (E. coli)
Chromosome 1
Common name neo; neomycin;
Symbol description No Data
References J Invest Dermatol. 2002 Sep;119(3):678-83.

Targeted disruption of dermatopontin causes abnormal collagen fibrillogenesis.
Research applications Cell Biology Research,
Dermatology Research
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
J. Invest. Dermatol., 119, 678-683 (2002).
Additional information
1 Lab HP
2 Genotyping protocol <PCR>
Depositor Nakayama, Toshinori (Graduate School of Medicine and School of Medicine, Chiba University) Nakayama, Toshinori
Strain Status /
Availability
(Expected delivery)


Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months