RBRC01474, B6.129-Fas<tm1Osa>/OsaRbrc

RBRC No. RBRC01474
Type Targeted MutationCartagena wks
Species Mus musculus
Strain name B6.129-Fas<tm1Osa>/OsaRbrc
Former Common name B6 Fas-KO
H-2 Haplotype No Data
Background strain No Data
Appearance
1 Appearance black
Genotype a/a B/B C/C
Strain development Developed by Drs. Masashi Adachi and Shigekazu Nagata at Osaka University Medical School in 1995.
Strain description Fas is a cell surface receptor belonging to the tumor necrosis factor receptor superfamily which transduces apoptotic signals in many cell types. Fas deficient mutant mice show lymphoma and autoimmune disorders. In a few month after birth, the mice develop lymphadenoma and splenomegaly by an accumulation of abnormal polyclonal T cells with the CD4-CD8- double negative.
Colony maintenance Backcross to C57BL/6 (Heterozygote x C57BL/6NCrlCrlj)
Homozygous mutant mice show lymphoma and autoimmune disorders. 50% dead at about 5 months of age.
Health Report
Gene Details
Promoter No Data
1 Symbol Fas
Symbol name Fas (TNF receptor superfamily member)
Chromosome 19
Common name APO-1, CD95, TNFR6, Tnfrsf6
Symbol description No Data
2 Symbol lacZ
Symbol name beta-galactosidase (E. coli)
Chromosome 19
Common name No Data
Symbol description E. coli beta galactosidase
Promoter mouse phosphoglycerate kinase promoter (PGK promoter)
3 Symbol neo
Symbol name neomycin resistance gene (E. coli)
Chromosome 19
Common name neo; neomycin;
Symbol description No Data
References Nat Genet. 1995 Nov;11(3):294-300.

Targeted mutation in the Fas gene causes hyperplasia in peripheral lymphoid organs and liver.
Research applications Immunology and Inflammation Research
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
Nature Genetics, 11, 294-300 (1995).
RECIPIENT may only use the BIOLOGICAL RESOURCE for non-commercial academic research purpose.
The RECIPIENT should negotiate with the DEPOSITOR in the case of application for any patents or commercial use with the results from the use of the BIOLOGICAL RESOURCE.
Additional information
1 Lab HP
2 Genotyping protocol <PCR>
Depositor Nagata, Shigekazu (Graduate School of Medicine, Kyoto University) Nagata, Shigekazu
Strain Status /
Availability
(Expected delivery)


Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months