RBRC01483, B6.Cg-Tg(Thy1-spH)10Yyan/Rbrc

RBRC No. RBRC01483
Type TransgeneCartagena
Species Mus musculus
Strain name B6.Cg-Tg(Thy1-spH)10Yyan/Rbrc
Former Common name TV-10, Thy1-SpH transgenic mice (TV-10)
H-2 Haplotype No Data
Background strain No Data
Appearance
1 Appearance black
Genotype a/a B/B C/C
Strain development Developed by Yuchio Yanagawa, Gunma University Graduate School of Medicine and Hiromu Yawo, Tohoku University Graduate School of Life Sciences in 2002-2003. Transgene was injected into the introduced into the pronuclei of B6D2F2 fertilized eggs. The Tg mice were crossed to C57BL/6J.
Strain description Vesicular exocytosis at presynaptic terminals is fundamental function for neurotransmission and brain function. The lumen of a secretory vesicle is acidic (pH 5.6), whereas it becomes neutral (pH 7.4) instantaneously upon exocytosis. The intravesicular change of pH can be detected by a fluorescence change of a pH-sensitive derivative of green fluorescent protein (pHluorin). SynaptopHluorin (SpH) is a fusion protein of pHluorin and syntaxin (VAMP2) for targeting to secretory vesicles. Thy1-SpH transgenic mouse expresses SpH in brain under the control of neuron-specific Thy1.2 promoter. This strain is useful for studies of presynaptic terminals in various regions of the brain. The spH mRNAs was expressed in whole brains of TV-7, TV-10, and TV-34 transgenic lines, whereas in TV-42, it was expressed in the specific regions of the hippocampus. Tg mice mice show no obvious abnormality.
Colony maintenance Carrier x Noncarrier [C57BL/6JJcl]
Health Report No Data
Gene Details
Promoter No Data
1 Symbol pHluorin
Symbol name pH-sensitive green fluorescent proteins, S147E, N149L, I161T, V163A, N164I, K166Q, I167V, R168H, S202H and L231H. (Jellyfish)
Chromosome UN
Common name No Data
Symbol description No Data
Promoter neuron-specific Thy-1.2 promoter
2 Symbol Vamp2
Symbol name vesicle-associated membrane protein 2
Chromosome UN
Common name Syb-2, Syb2, synaptobrevin II
Symbol description No Data
References Genesis 2005 Jun;42(2):53-60.

Transgenic mouse lines expressing synaptopHluorin in hippocampus and cerebellar cortex.
Research applications Fluorescent Proteins/lacZ System
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
Araki, R., Sakagami, H., Yanagawa, Y., Hikima, T., Ishizuka, T., and Yawo, H. Transgenic mouse lines expressing synaptopHluorin in hippocampus and cerebellar cortex. Genesis 42, 53-60 (2005).
In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
1) The Recipient should use the Material for research studies or education only in his laboratory.
2) The Material should be used with prudence and appropriate caution, since not all characteristics of the Material are fully known.
3) The Material should not be used in humans.
4) If the use of this material results in one or more scientific publications, Dr. Hiromu Yawo and myself (Yuchio Yanagawa) should be included as co-authors at least in the first paper.
5) The Material should not be redistributed without my permission.
6) The Material should not be used for commercial purpose.
Additional information
1 Mouse of the Month Sep 2006
2 GFP Transfer License (Japanese / English)
Please fill in the Schedule A, and submit two signed copies to us together with two signed copies of RIKEN BRC’s MTA. Please also read Schedule B.
3 Genotyping protocol <PCR>
Depositor Yanagawa, Yuchio (Gunma university) Yanagawa, Yuchio
Strain Status /
Availability
(Expected delivery)


Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months