RBRC01494, B6;129S2-Dppa3<tm1(rtTA)Nkn>/NknRbrc

RBRC No. RBRC01494
Type Targeted MutationCartagena
Species Mus musculus
Strain name B6;129S2-Dppa3<tm1(rtTA)Nkn>/NknRbrc
Former Common name PGC7 KO
H-2 Haplotype No Data
ES cell line D3 [129S2/SvPas]
Donor strain 129S2/SvPas via D3 ES cell line
Background strain No Data
Appearance
1 Appearance No Data
Genotype No Data
Strain development Developed by Toshinobu Nakamura, Toru Kimura and Toru Nakano, Graduate School of Medicine and Frontier Biosciences, Osaka University in 2003. D3 ES cells were used to generate the mutant mice. The mice were crossed to C57BL/6 (C57BL/6 semicongenic).
Strain description Dppa3 (PGC7/Stella) gene knockout (rtTA knock-in mice). The start codon of Dppa3 was replaced with rtTA cDNA and floxed PGK-neo cassette. Dppa3 gene plays a critical role in embryonic development as a maternal facotor. Homozygote males mice are fully fertile, but homozygous females are infertile.
Colony maintenance Heterozygote x Wild-type [C57BL/6NCrSlc]
Health Report No Data
Gene Details
Promoter No Data
1 Symbol Dppa3
Symbol name developmental pluripotency-associated 3
Chromosome 6
Common name 2410075G02Rik, PGC7, stella
Symbol description No Data
2 Symbol rtTA
Symbol name E. coli Reverse tetracycline transactivator (Mutated tetrepressor DNA-binding domain and the transactivation domain of VP-16)
Chromosome 6
Common name E. coli tet repressor (TetR), herpes simplex virus (HSV)VP16 activation domain
Symbol description No Data
3 Symbol loxP
Symbol name phage P1 loxP
Chromosome 6
Common name No Data
Symbol description No Data
Promoter mouse phosphoglycerate kinase promoter (PGK promoter)
4 Symbol neo
Symbol name neomycin resistance gene (E. coli)
Chromosome 6
Common name neo; neomycin;
Symbol description No Data
References Unpublished

Research applications Cre/loxP system,
Tet system
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR.
The RECIPIENT must inform the DEPOSITOR the research project using the BIOLOGICAL RESOURCE.
Additional information
1 Lab HP (Japanese)
2 Genotyping protocol <PCR>
Depositor Nakamura, Toshinobu (Nagahama Institute of Bio-Science and Techology) Nakamura, Toshinobu
Strain Status /
Availability
(Expected delivery)


Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months