RBRC01512, B6.129-Mknk1<tm1Fuku>/FukuRbrc

RBRC No. RBRC01512
Type Targeted MutationCartagena wks
Species Mus musculus
Strain name B6.129-Mknk1<tm1Fuku>/FukuRbrc
Former Common name Mnk1-KO, Mnk1 knockout mouse
H-2 Haplotype No Data
ES cell line R1 [(129X1/SvJ x 129S1/Sv)F1-Kitl<+>]
Donor strain 129X1/SvJ x 129S1/Sv)F1 via R1 ES cell line
Background strain No Data
Appearance
1 Appearance No Data
Genotype No Data
Strain development Developed by Rikiro Fukunaga, Graduate School of Frontier Biosciences, Osaka University in 2003. A neomycin selection cassette replaced a fragment from 5 to 6 exons of the Mnk1 and a fragment of 5 to 9 exons of the Mnk1, respectively. R1 ES cells were used. The mutant mice are crossed to C57BL/6.
Strain description Mnk1 gene knockout mice (RBRC01512), Mnk2 gene knockout mice (RBRC01513), Mnk1 and Mnk2 genes double knockout mice (RBRC01514). Mnk1 and Mnk2 (MAP kinase-interacting serine/threonine kinase 1 and 2) genes are protein kinases that are directly phosphorylated and activated by extracellular signal-regulated kinase or p38 mitogen-activated protein kinases. These genes are essential for constitutive and inducible phosphorylation of eukaryotic initiation factor 4E, but not for cell growth or development. This strain is useful for elucidation of the role of the functions of Mnk1 and Mnk2 genes. Mnk1 homozygous, Mnk2 homozygous and Mnk1/Mnk2 double homozygous knockout mice are viable and fertile.
Colony maintenance Homozygote x Homozygote [or Crossing to C57BL/6NCrlCrlj]
Health Report
Gene Details
Promoter No Data
1 Symbol Mknk1
Symbol name MAP kinase-interacting serine/threonine kinase 1
Chromosome 4
Common name 2410048M24Rik, Mnk1
Symbol description No Data
Promoter mouse phosphoglycerate kinase promoter (PGK promoter)
2 Symbol neo
Symbol name neomycin resistance gene (E. coli)
Chromosome 4
Common name neo; neomycin;
Symbol description No Data
References Mol Cell Biol 2004 Aug;24(15):6539-49.

Mnk2 and Mnk1 are essential for constitutive and inducible phosphorylation of eukaryotic initiation factor 4E but not for cell growth or development.
Research applications Cell Biology Research
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
Mol. Cell. Biol., 24, 6539-6549 (2004).
RECIPIENT may only use the BIOLOGICAL RESOURCE for non-commercial academic research purpose.
The RECIPIENT should negotiate with the DEPOSITOR in the case of application for any patents or commercial use with the results from the use of the BIOLOGICAL RESOURCE.
Additional information
1 Contact Information (Japanese)
2 Genotyping protocol <PCR>
Depositor Fukunaga, Rikiro (Kyoto University) Fukunaga, Rikiro
Strain Status /
Availability
(Expected delivery)


Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months