RBRC01518, D2;B6CB-Trp53<tm1Sia>/TyagRbrc

RBRC No. RBRC01518
Type Targeted MutationCartagena wks
Species Mus musculus
Strain name D2;B6CB-Trp53<tm1Sia>/TyagRbrc
Former Common name p53 targeting mice DBA/2 backcross N7; p53 KO DBA/2 back cross mouse
H-2 Haplotype No Data
ES cell line TT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1]
Donor strain (C57BL/6 x CBA)F1 via TT2 ES cell line
Background strain No Data
Appearance
1 Appearance dilute brown
Genotype a/a b/b C/C d/d
Strain development Developed by Shinichi Aizawa, RIKEN Tsukuba Institute. The TT2 ES cells derived from B6CBAF1 were used to disrupt the p53 gene by homologous recombination. The mutant mice were backcrossed to C57BL/6, ICR and C3H, respectively by Ohtura Niwa, Radiation Biology Center, Kyoto University, to DBA/2 by Takeshi Yagi, Graduate School of Frontier Biosciences, Osaka University, to MSM by Ryo Kominami, Niigata University Graduate School of Medical and Dental Sciences.
Strain description Trp53 gene knockout mice. A neomycin selection cassette was inserted into the second exon of the Trp53 gene. Homozygous deficient mice can grow after birth, but often suffered from development of tumors in various region of the body. Several congenic strains were generated. C57BL/6 background (RBRC01361), ICR background (RBRC01348), C3H background (RBRC00107), DBA/2 background (RBRC01518), and MSM background (RBRC00815). In C57BL/6 background most of the female homozygotes die in utero (probably, also in MSM background).
Colony maintenance Heterozygote x Wild-type [or Crossing to DBA/2NCrlCrlj]
Health Report
Gene Details
Promoter No Data
1 Symbol Trp53
Symbol name transformation-related protein 53
Chromosome 11
Common name p53
Symbol description No Data
Promoter herpes simplex virus thymidine kinase promoter (HSV tk promoter)
2 Symbol neo
Symbol name neomycin resistance gene (E. coli)
Chromosome 11
Common name neo; neomycin;
Symbol description No Data
References Oncogene. 1993 Dec;8(12):3313-22.

Enhanced proliferative potential in culture of cells from p53-deficient mice.
Research applications Cancer Research,
Cell Biology Research,
Immunology and Inflammation Research,
Mouse Models for Human Disease
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
Oncogene, 8, 3313-3322 (1993).
Please inform Laboratory for Animal Resources and Genetic Engineering,
CDB (e mail: mutant@cdb.riken.jp) about the paper when published.
Additional information
1 Breeding characters
2 Lab HP (Japanese)
3 Genotyping protocol <PCR>
Depositor Yagi, Takeshi (Graduate School of Frontier Biosciences, Osaka University) Yagi, Takeshi
Strain Status /
Availability
(Expected delivery)


Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months