RBRC01544, B6.129P2-Sirpa<tm1Nog>/Rbrc

RBRC No. RBRC01544
Type Targeted MutationCartagena wks
Species Mus musculus
Strain name B6.129P2-Sirpa<tm1Nog>/Rbrc
Former Common name SHPS-1 Knockout mice
H-2 Haplotype No Data
ES cell line E14.1 [129P2/OlaHsd]
Donor strain 129P2/OlaHsd via E14.1 ES cell line
Background strain C57BL/6NJcl
1 Appearance black
Genotype No Data
Strain development Developed by Dr. Kenjiro Inagaki, Kobe University School of Medicine in 2000. The construct was electoroporated into E14.1 ES cells derived from 129P2/OlaHsd. The mutant mice were backcrossed C57BL/6N over 10 times.
Strain description SHPS-1 knockout mice. Exon 7 and 8 were replaced with a nemoycin selection cassette. SHPS-1 (Ptpns1, protein tyrosine phosphatase, non-receptor type substrate 1) gene ia a member of sinal-regulatory proteins, plays a impotant roles in integrin-mediated cytoskeletal reoganization, cell motility and the regulation of Rho.
Colony maintenance Heterozygote x Wild-type [C57BL/6NJcl]. Homozygous mutant mice are viable and fertile.
Health Report
Gene Details
Promoter No Data
1 Symbol Sirpa
Symbol name signal-regulatory protein alpha
Chromosome 2
Common name Bit, CD172a, P84, SHPS-1, SIRP; Ptpns1;protein tyrosine phosphatase, non-receptor type substrate 1
Symbol description No Data
Promoter mouse phosphoglycerate kinase promoter (PGK promoter)
2 Symbol neo
Symbol name neomycin resistance gene (E. coli)
Chromosome 2
Common name neo; neomycin;
Symbol description No Data
References EMBO J 2000 Dec 15;19(24):6721-31.

SHPS-1 regulates integrin-mediated cytoskeletal reorganization and cell motility.
Research applications No Data
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR.
The RECIPIENT of BIOLOGICAL RESOURCE must submit its receipt to the DEPOSITOR immediately after receiving the BIOLOGICAL RESOURCE.
Additional information
1 Genetic Background
2 Lab HP
3 Genotyping protocol <PCR>
Depositor Matozaki, Takashi (Kobe University) Matozaki, Takashi
Strain Status /
(Expected delivery)

Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months
BRC mice in Publications
Title Journal
Signal Inhibitory Regulatory Protein Alpha Influences Macrophage Clearance From The Alveolar Space After An Inflammatory Stimulus. Am. J. Respir. Crit. Care Med.181: A2761 (2010).
Mark Kearns, William J. Janssen, and Peter M. Henson.
SHPS-1 deficiency induces robust neuroprotection against experimental stroke by attenuating oxidative stress. J Neurochem.122(4): 834-843 (2012).(22671569)
Wang L, Lu Y, Deng S, Zhang Y, Yang L, Guan Y, Matozaki T, Ohnishi H, Jiang H, Li H.
Increased Susceptibility to Salmonella Infection in Signal Regulatory Protein alpha-Deficient Mice. J Immunol.189(5): 2537-44 (2012).(22851710)
Li LX, Atif SM, Schmiel SE, Lee SJ, McSorley SJ.