RBRC01593, B6.129P2-Bcl2<tm1Tsu>/TsuRbrc

RBRC No. RBRC01593
Type Targeted MutationCartagena wks
Species Mus musculus
Strain name B6.129P2-Bcl2<tm1Tsu>/TsuRbrc
Former Common name bcl-2 knockout mouse
H-2 Haplotype No Data
ES cell line E14 [129P2/OlaHsd]
Donor strain 129P2/OlaHsd via E14 ES cell line
Background strain No Data
Appearance
1 Appearance No Data
Genotype No Data
Strain development Developed by Drs. Kamada Shinji and Yoshihide Tsujimoto at Osaka University Medical School in 1994. A neomycin selection cassette replaced most of coding sequences of exons 2 of the Bcl2 gene. The mutant mice were backcrossed C57BL/6J.
Strain description B6.129P2-Bcl2<tm1Tsu>. Bcl2, B-cell leukemia/lymphoma 2 gene is a major regulator of programmed cell death, initially identified through study of the t(14;18) chromosomal translocation in human B cell follicular lymphomas. This gene is expressed in variety of tissues and cell types in embryos and adults. Most of homozygous mutant mice displays growth retardation, smaller ears, short lives, polycystic kidney, atrophic thymus and sleep with accelerated apoptotic cell death of lymphocyes, and hair hypopigmentaion in the second hair follicle cycle.
Colony maintenance Backcross to C57BL/6 (Heterozygote x C57BL/6JJcl)
Health Report
BRC facility Health Report in the last year and a half

Examination Date Room Rack
Jul 8, 2019 2-検 F
May 7, 2019 2-検 F
Mar 11, 2019 2-検 F
Jan 15, 2019 2-検 F
Nov 12, 2018 2-検 F
Sep 10, 2018 2-検 F
Jul 9, 2018 2-検 F
May 14, 2018 2-検 F

Gene Details
Promoter No Data
1 Symbol Bcl2
Symbol name B-cell leukemia/lymphoma 2
Chromosome 1
Common name Bcl-2, C430015F12Rik, D630044D05Rik, D830018M01Rik
Symbol description No Data
Promoter mouse phosphoglycerate kinase promoter (PGK promoter)
2 Symbol neo
Symbol name neomycin resistance gene (E. coli)
Chromosome 1
Common name neo; neomycin;
Symbol description No Data
References Cancer Res. 1995 Jan 15;55(2):354-9.

bcl-2 deficiency in mice leads to pleiotropic abnormalities: accelerated lymphoid cell death in thymus and spleen, polycystic kidney, hair hypopigmentation, and distorted small intestine.
Research applications No Data
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
Kamada S, Shimono A, Shinto Y, Tsujimura T, Takahashi T, Noda T, Kitamura Y, Kondoh H, Tsujimoto Y. bcl-2 deficiency in mice leads to pleiotropic abnormalities: accelerated lymphoid cell death in thymus and spleen, polycystic kidney, hair hypopigmentation, and distorted small intestine. Cancer Res. 1995 Jan 15;55(2):354-9.
In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the indicated literature and an acknowledgment to the DEPOSITOR is requested. The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR/DEVELOPER.
Additional information
1 No Data
2 Genotyping protocol <PCR>
Depositor Yoshihide, Tsujimoto (Osaka University Medical School and Graduate school of Frontier Biosciences) Yoshihide, Tsujimoto
Strain Status /
Availability
(Expected delivery)


Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months