RBRC01601, B6.129S7-Tert<tm1Fish>/FishRbrc

RBRC No. RBRC01601
Type Targeted Mutation CongenicCartagena wks
Species Mus musculus
Strain name B6.129S7-Tert<tm1Fish>/FishRbrc
Former Common name mTERT KO mice
H-2 Haplotype No Data
ES cell line JH1 [129S7/SvEvBrd]
Donor strain 129S7/SvEvBrd via JH1 ES cell line
Background strain C57BL/6NCrlCrlj
Appearance
1 Appearance black
Genotype a/a B/B C/C
Strain development Developed by Drs. Xunmei Yuan and Fuyuki Ishikawa at Tokyo Institute of Technology in 1999. A neomycin selection cassette replaced three exons, encoding a highly conserved motif A of the Tert gene. The mutant mice were backcrossed C57BL/6N.
Strain description Tert, telomerase reverse transcriptase gene encodes a catalytic subunit of telomerase that is essential for telomerase activity. Homozygous mutant mice show no telomerase activity. Homozygous mutant mice after 3 to 5 generations were sterile.
Colony maintenance Backcross to C57BL/6 (Heterozygote x C57BL/6NCrlCrlj)
Homozygous mutant mice after 3 to 5 generations were sterile.
Health Report
Gene Details
Promoter No Data
1 Symbol Tert
Symbol name telomerase reverse transcriptase
Chromosome 13
Common name EST2, TCS1, TP2
Symbol description No Data
2 Symbol No Data
Symbol name bovine growth hormone polyA signal
Chromosome 13
Common name No Data
Symbol description No Data
Promoter mouse RNA polymenase II promoter, bovine growth hormone poly A signal
3 Symbol neo
Symbol name neomycin resistance gene (E. coli)
Chromosome 13
Common name neo; neomycin;
Symbol description No Data
References Genes Cells. 1999 Oct;4(10):563-72.

Presence of telomeric G-strand tails in the telomerase catalytic subunit TERT knockout mice.
Research applications No Data
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
Genes to Cells, 4, 563-572 (1999).
In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
The Recipient agrees to use the Biological Resource for academic use, shall not use for commercial use.
Additional information
1 Lab HP (Japanese)
2 Genotyping protocol <PCR>
Depositor Ishikawa, Fuyuki (Kyoto University) Ishikawa, Fuyuki
Strain Status /
Availability
(Expected delivery)


Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months