RBRC01663, B6D2-Tg(hK14-AhR-CA)239Mym/MymRbrc

RBRC No. RBRC01663
Type Transgene CongenicCartagena wks
Species Mus musculus
Strain name B6D2-Tg(hK14-AhR-CA)239Mym/MymRbrc
Former Common name K14-AhR-CA TG mouse line 239
H-2 Haplotype No Data
Background strain Jcl:B6D2F1
Appearance
1 Appearance black
Genotype a/a B/? C/C D/?
2 Appearance brown
Genotype a/a b/b C/C D/D
3 Appearance dilute brown
Genotype a/a b/b C/C d/d
4 Appearance dilute black
Genotype a/a B/? C/C d/d
Strain development Developed by Dr. Masayuki Yamamoto at Tsukuba University in 2002. A cDNA fragment encording the constitutive active form of mouse AhR (AhR-CA) under the human cytokeratin 14 promoter was injected into the pronuclei of fertilized egges of B6D2F1.
Strain description K14-AhR-CA transgenic mice carry a cDNA fragment encording the constitutive active form of mouse AhR (aryl hydrocarbon receptor) under control of human cytokeratin 14 promoter. The aryl hydrocarbon receptor (AhR) (dioxin receptor) is a transcription factor that mediate to catalyze xenobiotic Phase I metabolism. Tg hemizygote mice are normal at birth, but severe skin lesions with itching develop postnatally. This strain is useful for the evaluation of the causal relationship between the AhR gene expression and inflammatory dermatitis.
Colony maintenance Cross to B6D2F1 (Hemizygous x Jcl:B6D2F1)
Tg hemizygote mice are normal at birth, but severe skin lesions with itching develop postnatally.
Health Report
BRC facility Health Report in the last year and a half

Examination Date Room Rack
May 7, 2012 2-検 A
Mar 5, 2012 2-検 A
Jan 10, 2012 2-検 A
Nov 7, 2011 2-検 A
Sep 5, 2011 2-検 A
Jul 4, 2011 2-検 A
Apr 25, 2011 2-検 A
Mar 7, 2011 2-検 A

Gene Details
Promoter No Data
1 Symbol HA
Symbol name human influenza virus HA (hemagglutinin) tag sequence
Chromosome No Data
Common name No Data
Symbol description No Data
2 Symbol poly A
Symbol name poly A
Chromosome No Data
Common name No Data
Symbol description No Data
Promoter human Keratin14 promoter
3 Symbol Ahr
Symbol name aryl-hydrocarbon receptor
Chromosome UN
Common name Ah, Ahh, Ahre, dioxin receptor, In
Symbol description No Data
References Mol Cell Biol. 2005 Nov;25(21):9360-8.

Constitutive expression of aryl hydrocarbon receptor in keratinocytes causes inflammatory skin lesions.
Research applications Dermatology Research
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
Mol. Cell. Biol., 25, 9360-9368 (2005).
In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
The RECIPIENT agrees to include the DEPOSITOR/DEVELOPER as a co-author in any publications resulted by the use of the BIOLOGICAL RESOURCE during the first 2 years after deposition by the DEPOSITOR to the RIKEN BRC. The RECIPIENT should contact the DEPOSITOR in the case of application for any patents or commercial use with the results from the use of the BIOLOGICAL RESOURCE.
Additional information
1 Mouse of the Month Aug 2007
2 Lab HP (Japanese)
3 Genotyping protocol <PCR>
Depositor Yamamoto, Masayuki (University of tsukuba) Yamamoto, Masayuki
Strain Status /
Availability
(Expected delivery)


Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months