RBRC01723, B6;129-Dffb<tm1Osa>/OsaRbrc

RBRC No. RBRC01723
Type Targeted MutationCartagena wks
Species Mus musculus
Strain name B6;129-Dffb<tm1Osa>/OsaRbrc
Former Common name CAD KO; CAD Knockout mouse
H-2 Haplotype No Data
Donor strain (129X1/SvJ x 129S1/Sv)F1-Kitl+ via R1 ES cell line
Background strain No Data
1 Appearance black
Genotype a/a B/B C/C
Strain development Developed by Drs. Kohki Kawane and Shigekazu Nagata at Osaka University in 2003. A neomycin cassette replaced exons 1-6 and part of 7 of Dffb gene. The mutant mice were backcrossed to C57BL/6.
Strain description B6;129-Dffb<tm1Osa>. CAD, Caspase-activated DNase is an endonuclease that is activated in apoptotic cells. CAD deficient thymocytes did not undergo apoptotic DNA fragmentation, but their DNA was digested by thymic macrophages that effectively phagocytosed the
apoptotic thymocytes. This strain is useful for elucidation of the function of CAD gene.
Colony maintenance Backcross to C57BL/6 (Heterozygote x C57BL/6NCrlCrlj)
Homozygous mutant mice show no gross developmental abnormalities and are fertile with normal fecundity.
Health Report No Data
Gene Details
Promoter No Data
1 Symbol Dffb
Symbol name DNA fragmentation factor, beta subunit
Chromosome 4
Common name 40kDa, 5730477D02Rik, caspase-activated DNase, CPAN, DFF40, Didff
Symbol description No Data
Promoter herpes simplex virus thymidine kinase promoter (HSV tk promoter)
2 Symbol neo
Symbol name neomycin resistance gene (E. coli)
Chromosome 4
Common name neo; neomycin;
Symbol description No Data
References Nat Immunol. 2003 Feb;4(2):138-44. Epub 2003 Jan 13.

Impaired thymic development in mouse embryos deficient in apoptotic DNA degradation.
Research applications No Data
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
Nat. immunol., 4, 138-144 (2003).
RECIPIENT may only use the BIOLOGICAL RESOURCE for non-commercial academic research purpose.
The RECIPIENT should negotiate with the DEPOSITOR in the case of application for any patents or commercial use with the results from the use of the BIOLOGICAL RESOURCE.
Additional information
1 Lab HP
2 Genotyping protocol <PCR>
Depositor Nagata, Shigekazu (Graduate School of Medicine, Kyoto University) Nagata, Shigekazu
Strain Status /
(Expected delivery)

Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months
BRC mice in Publications
Title Journal
Novel role for caspase-activated DNase in the regulation of pathological cardiac hypertrophy. Hypertension65(4): 871-81 (2015).(25646292)
Gao L, Huang K, Jiang DS, Liu X, Huang D, Li H, Zhang XD, Huang K.