RBRC01725, B6;129-Dnase2a<tm1Osa>/OsaRbrc

RBRC No. RBRC01725
Type Targeted MutationCartagena wks
Species Mus musculus
Strain name B6;129-Dnase2a<tm1Osa>/OsaRbrc
Former Common name DNase II Hetero; DNase II Hetero Knockout mouse
H-2 Haplotype No Data
ES cell line R1 [(129X1/SvJ x 129S1/Sv)F1-Kitl<+>]
Donor strain (129X1/SvJ x 129S1/Sv)F1-Kitl+ via R1 ES cell line
Background strain No Data
1 Appearance black
Genotype a/a B/B C/C
Strain development Developed by Drs. Kohki Kawane and Shigekazu Nagata at Osaka University in 2001. A neomycin cassette replaced exons 1-5 and part of 6 of the Dnase2a gene. The mutant mice were backcrossed to C57BL/6.
Strain description B6;129-Dnase2a<tm1Osa>. In macrophage, DNase II, deoxyribonuclease II is responsible for digesting nuclear DNA expelled from erythroid precuursor cells. Homozygous null mice for DNase II are embryonic lethal due to sever anemia, and impaired definitive erythropoiesis in the fetal liver. This strain is useful for elucidation of the function of DNase II gene.
Colony maintenance Backcross to C57BL/6 (Heterozygote x C57BL/6NCrlCrlj)
Health Report No Data
Gene Details
Promoter No Data
1 Symbol Dnase2a
Symbol name deoxyribonuclease II alpha
Chromosome 8
Common name No Data
Symbol description No Data
2 Symbol loxP
Symbol name phage P1 loxP
Chromosome 8
Common name No Data
Symbol description No Data
Promoter mouse phosphoglycerate kinase promoter (PGK promoter)
3 Symbol neo
Symbol name neomycin resistance gene (E. coli)
Chromosome 8
Common name neo; neomycin;
Symbol description No Data

Research applications Mouse Models for Human Disease
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
Science, 292, 1546-1549 (2001).
RECIPIENT may only use the BIOLOGICAL RESOURCE for non-commercial academic research purpose.
The RECIPIENT should negotiate with the DEPOSITOR in the case of application for any patents or commercial use with the results from the use of the BIOLOGICAL RESOURCE.
For period of two (2) years after deposition by the DEPOSITOR to the REKEN BRC, the RECIPIENT agrees to include the DEPOSITOR/DEVELOPER as a co-author.
Additional information
1 Lab HP
2 Genotyping protocol <PCR>
Depositor Nagata, Shigekazu (Graduate School of Medicine, Kyoto University) Nagata, Shigekazu
Strain Status /
(Expected delivery)

Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months
BRC mice in Publications
Title Journal
STING manifests self DNA-dependent inflammatory disease. Proc Natl Acad Sci U S A. (2012).(23132945)
Ahn J, Gutman D, Saijo S, Barber GN.
DNase II-dependent DNA digestion is required for DNA sensing by TLR9. Nat Commun6: 5853 (2015).(25600358)
Chan MP, Onji M, Fukui R, Kawane K, Shibata T, Saitoh S, Ohto U, Shimizu T, Barber GN, Miyake K.