RBRC01733, B6.129P2-Ptafr<tm1Tksh>/TkshRbrc

RBRC No. RBRC01733
Type Targeted MutationCartagena wks
Species Mus musculus
Strain name B6.129P2-Ptafr<tm1Tksh>/TkshRbrc
Former Common name PAFR-KO, Platelet-activating factor (PAF) receptor knockout mouse
H-2 Haplotype No Data
ES cell line E14.1 [129P2/OlaHsd]
Donor strain 129P2/OlaHsd via E14.1 ES cell line
Background strain C57BL/6NCrlCrlj
Appearance
1 Appearance black
Genotype a/a B/B C/C
Strain development Developed by Dr. Satoshi Ishii at the University of Tokyo in 1998. A neomycin cassette was inserted into the open reading frame of Ptafr gene. The mutant mice were backcrossed to C57BL/6.
Strain description Platelet-activating factor (PAF) is a phospholipid mediator with pleiotropic and potent biological effects on a variety of cells and tissues. Homozygous mutant mice showed decreased susceptibility to bacterial infection. This strain is useful to elucidating the pathophysiological roles of PAF and PAFR in vivo.
Colony maintenance Backcross to C57BL/6 (Heterozygote x C57BL/6NCrlCrlj)
Health Report
Gene Details
Promoter No Data
1 Symbol Ptafr
Symbol name platelet-activating factor receptor
Chromosome 4
Common name PAF receptor, PAFR
Symbol description No Data
Promoter mouse phosphoglycerate kinase promoter (PGK promoter)
2 Symbol neo
Symbol name neomycin resistance gene (E. coli)
Chromosome 4
Common name neo; neomycin;
Symbol description No Data
References J Exp Med. 1998 Jun 1;187(11):1779-88.

Impaired anaphylactic responses with intact sensitivity to endotoxin in mice lacking a platelet-activating factor receptor.
Research applications No Data
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
J. Exp. Med., 187, 1779-1788 (1998).
Additional information
1 Lab HP (Japanese)
2 Genotyping protocol <PCR>
Depositor Shimizu, Takao (The University of Tokyo, Graduate School of Medicine) Shimizu, Takao
Strain Status /
Availability
(Expected delivery)


Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months
BRC mice in Publications
Title Journal
(PMID)
Author
Stimulation of Ly-6G on neutrophils in LPS-primed mice induces platelet-activating factor (PAF)-mediated anaphylaxis-like shock. J Leukoc Biol. (2011).(22131343)
Tanaka Y, Nagai Y, Kuroishi T, Endo Y, Sugawara S.
Platelet-activating factor receptor knockout mice are protected from MPTP-induced dopaminergic degeneration. Neurochem Int. (2013).(23743065)
Kim BK, Shin EJ, Kim HC, Chung YH, Dang DK, Jung BD, Park DH, Wie MB, Kim WK, Shimizu T, Nabeshima T, Jeong JH.