RBRC01828, B6.Cg-Tg(CAG-Cre)CZ-MO2Osb

RBRC No. RBRC01828
Type Transgene CongenicCartagena
Species Mus musculus
Strain name B6.Cg-Tg(CAG-Cre)CZ-MO2Osb
Former Common name CAG-cre
H-2 Haplotype No Data
Background strain C57BL/6
1 Appearance black
Genotype a/a B/B C/C
Strain development Developed by Masaru Okabe, Research Institute for Microbial Diseases, Osaka University in 2003. The transgene was injected into the pronuclei of B6D2F2 fertilized eggs. The mice were backcrossed C57BL/6N.
Strain description These transgenic mice express Cre recombinase gene under the control of CAG promoter. This strain can be used to make an inducible knock-out by crossing with the other genetically-engineered mice that have a loxP site-flanked sequence of interest. The recombination occurs in all over the body.
Colony maintenance Carrier x Noncarrier [C57BL/6NCrSlc]
Health Report
Gene Details
Promoter CAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA)
1 Symbol cre
Symbol name Phage P1 Cre recombinase
Chromosome UN
Common name No Data
Symbol description No Data
References Biochem Biophys Res Commun. 2004 Aug 20;321(2):275-9.

Lineage-specific cell disruption in living mice by Cre-mediated expression of diphtheria toxin A chain.
Research applications Cre/loxP system
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
Biochem. Biophys. Res. Commun., 321, 275-279 (2004).
RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or not-for-profit research is requested to enter into a Material Transfer Agreement with Osaka University (http://www.uic.osaka-u.ac.jp/en/index.html). The RECIPIENT which wants to use the BIOLOGICAL RESOURCE even after five years must obtain a written consent from the DEPOSITOR again.
Additional information
1 Genetic Background
2 Lab HP
3 Genotyping protocol <PCR>
Depositor Okabe, Masaru (Osaka university) Okabe, Masaru
Strain Status /
(Expected delivery)

Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months

Live mouse :
BRC mice in Publications
Title Journal
Clonal differentiation of skeletal muscle-derived CD34(-)/45(-) stem cells into cardiomyocytes in vivo. Stem Cells Dev.19(4): 503-12 (2010).(19634996)
Tamaki T, Uchiyama Y, Okada Y, Tono K, Masuda M, Nitta M, Hoshi A, Akatsuka A.
Hyaluronan deficiency in tumor stroma impairs macrophage trafficking and tumor neovascularization. Cancer Res.70(18): 7073-83 (2010).(20823158)
Kobayashi N, Miyoshi S, Mikami T, Koyama H, Kitazawa M, Takeoka M, Sano K, Amano J, Isogai Z, Niida S, Oguri K, Okayama M, McDonald JA, Kimata K, Taniguchi S, Itano N.
Decrease in topoisomerase I is responsible for activation-induced cytidine deaminase (AID)-dependent somatic hypermutation. Proc Natl Acad Sci U S A.108(48): 19305-10 (2011).(22080610)
Kobayashi M, Sabouri Z, Sabouri S, Kitawaki Y, Pommier Y, Abe T, Kiyonari H, Honjo T.
Basement membrane assembly of the integrin alpha 8 beta1 ligand nephronectin requires Fraser syndrome-associated proteins. J Cell Biol.197(5): 677-89 (2012).(22613833)
Kiyozumi D, Takeichi M, Nakano I, Sato Y, Fukuda T, Sekiguchi K.
Molecular Identification of t(w5): Vps52 Promotes Pluripotential Cell Differentiation through Cell-Cell Interactions. Cell Rep. (2012).(23142660)
Sugimoto M, Kondo M, Hirose M, Suzuki M, Mekada K, Abe T, Kiyonari H, Ogura A, Takagi N, Artzt K, Abe K.
CIZ1, a p21<sup>Cip1/Waf1</sup>-interacting protein, functions as a tumor suppressor in vivo. FEBS Lett. (2013).(23583447)
Nishibe R, Watanabe W, Ueda T, Yamasaki N, Koller R, Wolff L, Honda ZI, Ohtsubo M, Honda H.
Impaired epidermal permeability barrier in mice lacking the Elovl1 gene responsible for very long-chain fatty acid production. Mol Cell Biol. (2013).(23689133)
Sassa T, Ohno Y, Suzuki S, Nomura T, Nishioka C, Kashiwagi T, Hirayama T, Akiyama M, Taguchi R, Shimizu H, Itohara S, Kihara A.
New BRAF knock-in mice provide a pathogenetic mechanism of developmental defects and a therapeutic approach in cardio-facio-cutaneous syndrome. Hum Mol Genet. (2014).(25035421)
Inoue SI, Moriya M, Watanabe Y, Miyagawa-Tomita S, Niihori T, Oba D, Ono M, Kure S, Ogura T, Matsubara Y, Aoki Y.
Single-cell imaging of caspase-1 dynamics reveals an all-or-none inflammasome signaling response. Cell Rep.8(4): 974-82 (2014).(25127135)
Liu T, Yamaguchi Y, Shirasaki Y, Shikada K, Yamagishi M, Hoshino K, Kaisho T, Takemoto K, Suzuki T, Kuranaga E, Ohara O, Miura M.
ALKR1275Q perturbs extracellular matrix, enhances cell invasion and leads to the development of neuroblastoma in cooperation with MYCN. OncogeneFeb 1. doi: 10.1038/onc.2015.519. (2016).(26829053)
Ueda T, Nakata Y, Yamasaki N, Oda H, Sentani K, Kanai A, Onishi N, Ikeda K, Sera Y, Honda ZI, Tanaka K, Sata M, Ogawa S, Yasui W, Saya H, Takita J, Honda H.
The protein phosphatase 6 catalytic subunit (Ppp6c) is indispensable for proper post-implantation embryogenesis. Mech. Dev. (2016).(26868000)
Ogoh H, Tanuma N, Matsui Y, Hayakawa N, Inagaki A, Sumiyoshi M, Momoi Y, Kishimoto A, Suzuki M, Sasaki N, Ohuchi T, Nomura M, Teruya Y, Yasuda K, Watanabe T, Shima H.
Disruption of the Sjögren-Larsson Syndrome Gene Aldh3a2 in Mice Increases Keratinocyte Growth and Retards Skin Barrier Recovery. J. Biol. Chem.291(22): 11676-88. (2016).(27053112)
Naganuma T, Takagi S, Kanetake T, Kitamura T, Hattori S, Miyakawa T, Sassa T, Kihara A.
Propagation of trimethylated H3K27 regulated by polycomb protein EED is required for embryogenesis, hematopoietic maintenance, and tumor suppression Proc. Natl. Acad. Sci. USA (2016).(27578866)
Ueda T, Nakata Y, Nagamachi A, Yamasaki N, Kanai A, Sera Y, Sasaki M, Matsui H, Honda ZI, Oda H, Wolff L, Inaba T, Honda H.
Mice with an Oncogenic HRAS Mutation are Resistant to High-Fat Diet-Induced Obesity and Exhibit Impaired Hepatic Energy Homeostasis. EBioMedicine (2017).(29254681)
Oba D, Inoue SI, Miyagawa-Tomita S, Nakashima Y, Niihori T, Yamaguchi S, Matsubara Y, Aoki Y.
CLICK: one-step generation of conditional knockout mice. BMC Genomics (2018).(29720086)
Miyasaka Y, Uno Y, Yoshimi K, Kunihiro Y, Yoshimura T, Tanaka T, Ishikubo H, Hiraoka Y, Takemoto N, Tanaka T, Ooguchi Y, Skehel P, Aida T, Takeda J, Mashimo T.
Loss of DDHD2, whose mutation causes spastic paraplegia, promotes reactive oxygen species generation and apoptosis. Cell Death Dis.9(8): 797 (2018).(30038238)
Maruyama T, Baba T, Maemoto Y, Hara-Miyauchi C, Hasegawa-Ogawa M, Okano HJ, Enda Y, Matsumoto K, Arimitsu N, Nakao K, Hamamoto H, Sekimizu K, Ohto-Nakanishi T, Nakanishi H, Tokuyama T, Yanagi S, Tagaya M, Tani K.
Laminin γ1 C-terminal Glu to Gln mutation induces early postimplantation lethality. Life Sci. Alliance. (2018).(30456378)
Kiyozumi D, Taniguchi Y, Nakano I, Toga J, Yagi E, Hasuwa H, Ikawa M, Sekiguchi K.