RBRC01834, C57BL/6-Tg(CAG-flpe)36Ito/ItoRbrc

RBRC No. RBRC01834
Type TransgeneCartagena
Species Mus musculus
Strain name C57BL/6-Tg(CAG-flpe)36Ito/ItoRbrc
Former Common name B6-Tg(CAG-FLPe)36; C57BL/6-Tg(CAG-FLPe)36Ito/ItoRbrc
H-2 Haplotype No Data
Background strain C57BL/6JJcl
1 Appearance black
Genotype a/a B/B C/C
Strain development Deposited by Hiroaki Kanki and Shigeyoshi Itohara, RIKEN Brain Science Institute in 2006. The transgene was injected into the pronuclei of C57BL/6 fertilized eggs. C57BL/6J background.
Strain description This transgenic mouse of C57BL/6 strain expresses the FLPe recombinase under the control of the CAG promoter. FLPe is a variant of the FLP recombinase with a higher enzymatic activity at 37 degrees C. The FLP-FRT system is similar to the cre-loxP system and is useful for more complex dual approaches that combine FLP with other recombinases, such as Cre and phi C31. This strain can be used to make an inducible knock-out by crossing with the other genetically-engineered mice that have a pair of FLP recombinase target (FRT) sequence flanking the genomic region of interest.
Colony maintenance Carrier x Noncarrier [C57BL/6JJcl]
Health Report
Gene Details
Promoter No Data
1 Symbol IRES
Symbol name internal ribosomal entry site (EMCV)
Chromosome UN
Common name internal ribosomal entry site from EMCV
Symbol description No Data
2 Symbol puro
Symbol name puromycin resistance gene (Streptomyces alboniger)
Chromosome UN
Common name No Data
Symbol description No Data
Promoter CAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA)
3 Symbol FLPe
Symbol name yeast flippase recombinase
Chromosome UN
Common name No Data
Symbol description No Data
References Exp Anim. 2006 Apr;55(2):137-41.

High-efficiency CAG-FLPe deleter mice in C57BL/6J background.
Research applications FLP/frt system,
General Purpose,
Genetics Research
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
Experimental Animals, 55, 137-141 (2006).
Additional information
1 Mouse of the Month Feb 2007
Genetic Background
2 Lab HP
3 Genotyping protocol <PCR>
Depositor Itohara, ShigeYoshi (RIKEN Center for Brain Science) Itohara, ShigeYoshi
Strain Status /
(Expected delivery)

Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months

Live mouse :
BRC mice in Publications
Title Journal
Hyaluronan deficiency in tumor stroma impairs macrophage trafficking and tumor neovascularization. Cancer Res.70(18): 7073-83 (2010).(20823158)
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Deletion of the Mint3/Apba3 gene in mice abrogates macrophage functions and increases resistance to lipopolysaccharide-induced septic shock. J Biol Chem.286(37): 32542-51 (2011).(21778228)
Hara T, Mimura K, Abe T, Shioi G, Seiki M, Sakamoto T.
Hair cycle resting phase is regulated by cyclic epithelial FGF18 signaling. J Invest Dermatol.132(5): 1338-45 (2012).(22297635)
Kimura-Ueki M, Oda Y, Oki J, Komi-Kuramochi A, Honda E, Asada M, Suzuki M, Imamura T.
Wnt5a-Ror2 signaling between osteoblast-lineage cells and osteoclast precursors enhances osteoclastogenesis. Nat Med.18(3): 405-12 (2012).(22344299)
Maeda K, Kobayashi Y, Udagawa N, Uehara S, Ishihara A, Mizoguchi T, Kikuchi Y, Takada I, Kato S, Kani S, Nishita M, Marumo K, Martin TJ, Minami Y, Takahashi N.
Distinct cellular pathways select germline-encoded and somatically mutated antibodies into immunological memory. J Exp Med. (2012).(23027924)
Kaji T, Ishige A, Hikida M, Taka J, Hijikata A, Kubo M, Nagashima T, Takahashi Y, Kurosaki T, Okada M, Ohara O, Rajewsky K, Takemori T.
Enhanced apoptosis by disruption of the STAT3-IkappaB- signaling pathway in epithelial cells induces Sj gren’s syndrome-like autoimmune disease. Immunity.38(3): 450-60 (2013).(23453632)
Okuma A, Hoshino K, Ohba T, Fukushi S, Aiba S, Akira S, Ono M, Kaisho T, Muta T.
Physiological functions of osteoblast lineage and T cell-derived RANKL in bone homeostasis. J Bone Miner Res. (2013).(24014480)
Fumoto T, Takeshita S, Ito M, Ikeda K.
Acquired deficiency of A20 results in rapid apoptosis, systemic inflammation, and abnormal hematopoietic stem cell function. PLoS One (2014).(24498102)
Nagamachi A, Nakata Y, Ueda T, Yamasaki N, Ebihara Y, Tsuji K, Honda Z, Takubo K, Suda T, Oda H, Inaba T, Honda H.
Generation of a Tlx1CreER-Venus knock-in mouse strain for the study of spleen development. Genesis. (2014).(25283275)
Nakahara R, Kawai Y, Oda A, Nishimura M, Murakami A, Azuma T, Kaifu T, Goitsuka R.
The histone demethylase fbxl11/kdm2a plays an essential role in embryonic development by repressing cell-cycle regulators. Mech Dev. (2014).(25463925)
Kawakami E, Tokunaga A, Ozawa M, Sakamoto R, Yoshida N.
Eomesodermin-expressing T-helper cells are essential for chronic neuroinflammation. Nat. Commun. (2015).(26436530)
Raveney BJ, Oki S, Hohjoh H, Nakamura M, Sato W, Murata M, Yamamura T.
Kidney-specific knockout of Sav1 in the mouse promotes hyper-proliferation of renal tubular epithelium through suppression of the Hippo pathway. J. Pathol. (2016).(26913567)
Kai T, Tsukamoto Y, Hijiya N, Tokunaga A, Nakada C, Uchida T, Daa T, Iha H, Takahashi M, Nomura T, Sato F, Mimata H, Ikawa M, Seto M, Matsuura K, Moriyama M.
Characterization of the role of sphingomyelin synthase 2 in glucose metabolism in whole-body and peripheral tissues in mice. Biochim. Biophys. Acta. (2016).(27151272)
Sugimoto M, Shimizu Y, Zhao S, Ukon N, Nishijima K, Wakabayashi M, Yoshioka T, Higashino K, Numata Y, Okuda T, Tamaki N, Hanamatsu H, Igarashi Y, Kuge Y.
Maintenance of the functional integrity of mouse hematopoiesis by EED and promotion of leukemogenesis by EED haploinsufficiency. Sci Rep. Jul 19;6:29454. doi: 10.1038/srep29454. (2016).(27432459)
Ikeda K, Ueda T, Yamasaki N, Nakata Y, Sera Y, Nagamachi A, Miyama T, Kobayashi H, Takubo K, Kanai A, Oda H, Wolff L, Honda Z, Ichinohe T, Matsubara A, Suda T, Inaba T, Honda H.
Acquired expression of Cbl(Q367P) in mice induces dysplastic myelopoiesis mimicking chronic myelomonocytic leukemia. Blood129(15): 2148-2160 (2017).(28209720)
Nakata Y, Ueda T, Nagamachi A, Yamasaki N, Ikeda KI, Sera Y, Takubo K, Kanai A, Oda H, Sanada M, Ogawa S, Tsuji K, Ebihara Y, Wolff L, Honda ZI, Suda T, Inaba T, Honda H.
Secreted Metalloproteinase ADAMTS-3 Inactivates Reelin. J. Neurosci.37(12): 3181-3191 (2017).(28213441)
Ogino H, Hisanaga A, Kohno T, Kondo Y, Okumura K, Kamei T, Sato T, Asahara H, Tsuiji H, Fukata M, Hattori M.
Thymic epithelial cell-specific deletion of Jmjd6 reduces Aire protein expression and exacerbates disease development in a mouse model of autoimmune diabetes. Biochem. Biophys. Res. Commun.489(1): 8-13 (2017).(28546003)
Yanagihara T, Tomino T, Uruno T, Fukui Y.
Germ cell-intrinsic requirement for the homeodomain transcription factor PKnox1/Prep1 in adult spermatogenesis. PLoS One (2018).(29293683)
Kawai Y, Oda A, Kanai Y, Goitsuka R.
KLRG1+ Effector CD8+ T Cells Lose KLRG1, Differentiate into All Memory T Cell Lineages, and Convey Enhanced Protective Immunity. Immunity (2018).(29625895)
Herndler-Brandstetter D, Ishigame H, Shinnakasu R, Plajer V, Stecher C, Zhao J, Lietzenmayer M, Kroehling L, Takumi A, Kometani K, Inoue T, Kluger Y, Kaech SM, Kurosaki T, Okada T, Flavell RA.
Zfat expression in ZsGreen reporter gene knock-in mice: Implications for a novel function of Zfat in definitive erythropoiesis. Int. J. Mol. Med. (2018).(30106088)
Tsunoda T, Doi K, Ishikura S, Luo H, Nishi K, Matsuzaki H, Koyanagi M, Tanaka Y, Okamura T, Shirasawa S.
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Kimura-Yoshida C, Mochida K, Nakaya MA, Mizutani T, Matsuo I.
Laminin γ1 C-terminal Glu to Gln mutation induces early postimplantation lethality. Life Sci. Alliance. (2018).(30456378)
Kiyozumi D, Taniguchi Y, Nakano I, Toga J, Yagi E, Hasuwa H, Ikawa M, Sekiguchi K.