RBRC01840, B6.Cg-Grin2d<tm1Mim>/MimRbrc

RBRC No. RBRC01840
Type Targeted MutationCartagena
Species Mus musculus
Strain name B6.Cg-Grin2d<tm1Mim>/MimRbrc
Former Common name GluRε4 KO mouse
H-2 Haplotype No Data
ES cell line TT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1]
Background strain C57BL/6
1 Appearance black
Genotype No Data
Strain development Developed by Kazutaka Ikeda and Masami Mishina, Brain Research Institute, Niigata University in 1995. A pgk-neo was transfered into TT2 ES cells to replace the exon encoding the M4 putative transmembrane segment of Grin2d gene. The mutant mice were backcrossed to C57BL/6N over 10 times.
Strain description GluR epsilon4 (Grin2d, glutamate receptor, ionotropic, NMDA2D (epsilon 4)) knockout mice. The Grin2d gene is one of the glutamate receptor channel subunit families forming NMDA receptor channel. The Grin2d homozygous mutant mice show a reduced spontaneous activity, but no significant difference in motor activity and anxiety tests when compared with normal mice. Reproductive performance and growth of them is normal.
Colony maintenance Heterozygote x Wild-type [C57BL/6NJcl]. Homozygous mutant mice are viable and fertile.
Health Report
Gene Details
Promoter No Data
1 Symbol Grin2d
Symbol name glutamate receptor, ionotropic, NMDA2D (epsilon 4)
Chromosome 7
Common name GluRepsilon4, NMDAR2D, NR2D
Symbol description No Data
Promoter mouse phosphoglycerate kinase promoter (PGK promoter)
2 Symbol neo
Symbol name neomycin resistance gene (E. coli)
Chromosome 7
Common name neo; neomycin;
Symbol description No Data

Research applications Neurobiology Research
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
Mol. Brain. Res., 33, 61-71 (1995).
In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
Prior to filing for a patent, or intellectual property or other rights based on results of research using the BIOLOGICAL RESOURCE, the RECIPIENT shall acquire the consent from the DEPOSITOR for such application. Use of the BIOLOGICAL RESOURCE shall be limited to a collaborative research. And Use of the BIOLOGICAL RESOURCE shall require co-authorship of the DEPOSITOR for TWO years after deposition of the BIOLOGICAL RESOURCE to the RIKEN BRC or for the first publication if no publication is made within the TWO years.
Additional information
1 Genetic Background
2 Lab HP
3 Genotyping protocol <PCR>
Depositor Mishina, Masayoshi (The University of Tokyo, Graduate School of Medicine) Mishina, Masayoshi
Strain Status /
(Expected delivery)

Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months
BRC mice in Publications
Title Journal
GluN2D Subunit-Containing NMDA Receptors Control Tissue Plasminogen Activator-Mediated Spatial Memory. J Neurosci.32(37): 12726-34 (2012).(22972996)
Obiang P, Macrez R, Jullienne A, Bertrand T, Lesept F, Ali C, Maubert E, Vivien D, Agin V.
Phenotypic characterization of orofacial movement topography in mutants with disruption of amino acid mechanisms: Glutamate N2A/B/D [GluR 1/2/4] subtypes and the GABA synthesizing enzyme GAD65. Neuroscience.250: 743-54 (2013).(23892010)
Tomiyama K, Kato R, Hara Y, Kobayashi M, Mishina M, Yanagawa Y, Kinsella A, Koshikawa N, Waddington JL.