RBRC01939, B6.129X1-Trpv4<tm1Msz>

RBRC No. RBRC01939
Type Targeted MutationCartagena
Species Mus musculus
Strain name B6.129X1-Trpv4<tm1Msz>
Former Common name TRPV4KO
H-2 Haplotype No Data
ES cell line RW4 [129X1/SvJ]
Background strain C57BL/6JJcl
1 Appearance black
Genotype a/a b/b C/C
Strain development Developed by Makoto Suzuki, Jichi Medical School in 2001. The construct was electoroporated into RW4 ES cells derived from 129X1/SvJ. The mutant mice were backcrossed to C57BL/6J.
Strain description TRPV4 (Trpv4, transient receptor potential cation channel, subfamily V, member 4) knockout mice. Exon 4 encodes the ankyrin-repeat domain was replaced with a neomycin resistance gene. The ion channel TRPV4 is a member of the TRP family, acts as a Ca2+-permeable cation channel in response to mechanical stress. Homozygous mutant mice are viable and fertile, but show a reduced responsiveness to pressure sensation and abnormal osmotic regulation.
Colony maintenance Heterozygote x Wild-type [C57BL/6JJcl]
Health Report
Gene Details
Promoter No Data
1 Symbol Trpv4
Symbol name transient receptor potential cation channel, subfamily V, member 4
Chromosome 5
Common name 0610033B08Rik, OTRPC4, Trp12, VR-OAC, VRL-2, VROAC
Symbol description No Data
Promoter mouse phosphoglycerate kinase promoter (PGK promoter)
2 Symbol neo
Symbol name neomycin resistance gene (E. coli)
Chromosome 5
Common name neo; neomycin;
Symbol description No Data
References Am J Physiol Cell Physiol 2003 Jul;285(1):C96-101.
J Biol Chem. 2003 Jun 20;278(25):22664-8. Epub 2003 Apr 13.

Impaired osmotic sensation in mice lacking TRPV4.
Impaired osmotic sensation in mice lacking TRPV4.
Research applications Sensorineural Research
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
J, Biol. Chem., 278, 22664-22668 (2003).
The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR (Dr. Makoto Suzuki). The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
Additional information
1 No Data
2 Genotyping protocol <PCR>
Depositor suzuki, makoto (Jichi Medical University) suzuki, makoto
Strain Status /
(Expected delivery)

Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months

Live mouse :
BRC mice in Publications
Title Journal
Basal cells express functional TRPV4 channels in the mouse nasal epithelium. Biochem Biophys Rep4: 169-174 (2015).(29124201)
Ueda T, Hoshikawa M, Shibata Y, Kumamoto N, Ugawa S.