Strain Data Sheet

RBRC01952

Strain Information

Image
BRC No.RBRC01952
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6;129S2-Lats1<tm1Noj>
Former Common nameLats1 heterozygous (He) mouse
H-2 Haplotype
ES Cell lineD3 [129S2/SvPas]
Background strain
Appearance
Strain developmentDeveloped by Norikazu Yabuta and Hiroshi Nojima, Research Institute for Microbial Diseases, Osaka University in 2004. D3 ES cells were used. The mutant mice were crossed to C57BL/6.
Strain descriptionLats1 gene knockout mice (deltaN type). Exon 1 containing a translation initiation codon was replaced with a PGK-neo cassette. Homozygous mutant mice are small. Homozygous mutant females cannot nurse pups due to lacking mammae.
Colony maintenanceHeterozygote x Wild-type [C57BL/6NCrSlc]
ReferencesJ. Cell Sci., 126, 508-520 (2013). 23230145

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Lats1large tumor suppressor10Lats1<tm1Noj>targeted mutation 1, Hiroshi Nojima

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
neoneomycin resistance gene (E. coli)10neo; neomycin;mouse phosphoglycerate kinase promoter (PGK promoter)

Ordering Information

Donor DNAmouse phosphoglycerate kinase promoter (PGK promoter), E. coli neo, mouse Lats1 genomic DNA
Research applicationCell Biology Research
Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. J CellSci., 126, 508-520 (2013).The RECIPIENT agrees to use of this BIOLOGICAL RESOURCE as a collaboration with the DEPOSITOR.
DepositorHiroshi Nojima (Osaka University)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Approval form (Japanese / English)
  2. Order form (Japanese / English)
  3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  4. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data