RBRC01966, B6;129S4-ERas<tm1Yam>

RBRC No. RBRC01966
Type Targeted MutationCartagena wks
Species Mus musculus
Strain name B6;129S4-ERas<tm1Yam>
Former Common name No Data
H-2 Haplotype No Data
ES cell line RF8 [129S4/SvJae]
Background strain No Data
1 Appearance No Data
Genotype No Data
Strain development Developed by Dr. Shinya Yamanaka at Nara Institute of Science and Technology in 2003. A beta-geo cassette was inserted into the ERas coding region. The mutant mice were backcross to C57BL/6J for 4 times.
Strain description ERas gene is important in the tumor-like grouwth properties of ES cells.
Homozygous mutant mice develop normally and are fertile.
Colony maintenance Sibling Mating (Homozygote x Homozygote)
Health Report
Gene Details
Promoter No Data
1 Symbol Eras
Symbol name ES cell-expressed Ras
Chromosome X
Common name ecat5, Ha-Ras2, HRAS2, HRasp
Symbol description No Data
2 Symbol IRES
Symbol name internal ribosomal entry site (EMCV)
Chromosome X
Common name internal ribosomal entry site from EMCV
Symbol description No Data
3 Symbol neo
Symbol name neomycin resistance gene (E. coli)
Chromosome X
Common name neo; neomycin;
Symbol description No Data
4 Symbol lacZ
Symbol name beta-galactosidase (E. coli)
Chromosome X
Common name No Data
Symbol description E. coli beta galactosidase
References Nature. 2003 May 29;423(6939):541-5.

Role of ERas in promoting tumour-like properties in mouse embryonic stem cells.
Research applications No Data
Specific Term and Conditions For inquiries please mail us at animal@brc.riken.jp.
Additional information
1 MATERIAL TRANSFER AGREEMENT (Japanese (Word) / English (Word)
2 Genotyping protocol <PCR>
Depositor Yamanaka, Shinya (Institute for frontier medical sciences, Kyoto university) Yamanaka, Shinya
Strain Status /
(Expected delivery)

Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months