RBRC02078, B6;129P2-Slp2<tm1Mitf>

RBRC No. RBRC02078
Type Targeted MutationCartagena
Species Mus musculus
Strain name B6;129P2-Slp2<tm1Mitf>
Former Common name Slp2-a KO mouse
H-2 Haplotype No Data
ES cell line E14 [129P2/OlaHsd]
Background strain No Data
Appearance
1 Appearance black
Genotype a/a B/B C/C
Strain development Developed by Chika Saegusa and Mitsunori Fukuda, RIKEN Brain Science Institute in 2006. E14 ES cells derived from 129P2/OlaHsd were used to generated the knockout mice. The mutant mice were crossed C57BL/6.
Strain description Slp2 gene knockout mice. Exon 1 of the Slp2 was replaced with a neo cassette. Slp (Synaptoagmin-like protein) is a Rab family of low-molecular-G protein, and known as a molecule implicated in the Rab27-dependent membrane transport. Homozygous mutant mice shows an abnormal gastric surface mucus cell morphology and reduced number of mucus granules.
Colony maintenance Heterozygote x Wild-type [C57BL/6JJcl]
Health Report No Data
Gene Details
Promoter No Data
1 Symbol Sytl2
Symbol name synaptotagmin-like 2
Chromosome 7
Common name Slp2, Slp2-a, Slp2-b, Slp2-c, Slp2-d
Symbol description No Data
Promoter mouse phosphoglycerate kinase promoter (PGK promoter)
2 Symbol neo
Symbol name neomycin resistance gene (E. coli)
Chromosome 7
Common name neo; neomycin;
Symbol description No Data
References Genes Cells. 2006 Jun;11(6):623-31.

Decreased basal mucus secretion by Slp2-a-deficient gastric surface mucous cells.
Research applications Cell Biology Research,
Dermatology Research,
Internal/Organ Research
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
Saegusa, C., Itohara S. and Fukuda. M.Role of Rab27B-binding protein Slp1 in pancreatic acinar cells. In preparation
In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
Permission of Dr. Mitsunori Fukuda is requried before use of the Slp1-KO mice.
Additional information
1 Lab HP
2 Genotyping protocol <PCR>
Depositor Fukuda, Mitsunori (The Institute of Physical and Chemical Research) Fukuda, Mitsunori
Strain Status /
Availability
(Expected delivery)


Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months