RBRC02095, B6.B6D2-Tg(Th-EGFP)21-31Koba

RBRC No. RBRC02095
Type Transgene CongenicCartagena
Species Mus musculus
Strain name B6.B6D2-Tg(Th-EGFP)21-31Koba
Former Common name B6.Cg-Tg(TH-GFP)21-31
H-2 Haplotype No Data
Background strain C57BL/6JJcl
1 Appearance black
Genotype a/a B/B C/C
Strain development Developed by Kazuto Kobayashi, Fukushima Medical University School of Medicine in 1998. The transgene was injected into the pronuclei of B6D2F2 fertilized eggs. The transgenic mice were backcrossed to C57BL/6J. Two lines were generated. RBRC02095 (commonly-used), RBRC03162.
Strain description The TH-GFP transgenic mouse was generated to express GFP in the majority of midbrain dopamine neurons under the control of the rat TH gene promoter. Dopamine is a neurotransmitter which mediates motor control, cognition, emotion, reward, and neuroendocrine functions. Dysfunction of dopamine-producing neurons in the midbrain is involved in some neurological and neuropsychiatric diseases such as Parkinson's disease and schizophrenia. These dopamine neurons express tyrosine hydroxylase (TH), a key enzyme of catecholamine biosynthesis. This transgenic mouse is useful for visualizing dopamine neurons to study the physiology and pathogenesis of dopamine neurons. Homozygous transgenic mice are lethal.
Colony maintenance Carrier x Noncarrier [C57BL/6JJcl]
Health Report
Gene Details
Promoter rat tyrosine hydroxylase (TH) promoter
1 Symbol GFP
Symbol name Green Fluorescent Protein (Jellyfish)
Chromosome UN
Common name No Data
Symbol description No Data
References J Neurochem. 2002 Jul;82(2):295-304.

Dynamics of tyrosine hydroxylase promoter activity during midbrain dopaminergic neuron development.
Research applications Fluorescent Proteins/lacZ System,
Neurobiology Research
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
J. Neurochem., 82, 295-304 (2002).
Additional information
1 Mouse of the Month May 2008
Genetic Background
2 GFP Transfer License (Japanese / English)
Please fill in the Schedule A, and submit two signed copies to us together with two signed copies of RIKEN BRC’s MTA. Please also read Schedule B.
Lab HP (Japanese)
3 Construct map
Transgene sequence
4 Genotyping protocol <PCR>
Depositor Kobayashi, Kazuto (Fukushima Medical University) Kobayashi, Kazuto
Strain Status /
(Expected delivery)

Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

Live mouse :
BRC mice in Publications
Title Journal
Sensory Input Regulates Spatial and Subtype-Specific Patterns of Neuronal Turnover in the Adult Olfactory Bulb. J Neurosci.31(32): 11587-11596 (2011).(21832189)
Sawada M, Kaneko N, Inada H, Wake H, Kato Y, Yanagawa Y, Kobayashi K, Nemoto T, Nabekura J, Sawamoto K.
Structural basis for serotonergic regulation of neural circuits in the mouse olfactory bulb. J Comp Neurol. (2014).(25234191)
Suzuki Y, Kiyokage E, Sohn J, Hioki H, Toida K.
Identification of a Vav2-dependent mechanism for GDNF/Ret control of mesolimbic DAT trafficking. Nat. Neurosci. (2015).(26147533)
Zhu S, Zhao C, Wu Y, Yang Q, Shao A, Wang T, Wu J, Yin Y, Li Y, Hou J, Zhang X, Zhou G, Gu X, Wang X, Bustelo XR, Zhou J.
Differential contribution of Ih to the integration of excitatory synaptic inputs in Substantia Nigra pars compacta and Ventral Tegmental Area dopaminergic neurons. Eur. J. Neurosci. (2015).(26354486)
Masi A,, Narducci R, Resta F, Carbone C, Kobayashi K, Mannaioni G.
Spatial distribution of synapses on tyrosine hydroxylase expressing juxtaglomerular cells in the mouse olfactory glomerulus. J. Comp. Neurol. (2016).(27864931)
Kiyokage E, Kobayashi K, Toida K.