|Former Common name||B6.Cg-Tg(TH-GFP)21-31|
|H-2 Haplotype||No Data|
|Genotype||a/a B/B C/C|
|Strain development||Developed by Kazuto Kobayashi, Fukushima Medical University School of Medicine in 1998. The transgene was injected into the pronuclei of B6D2F2 fertilized eggs. The transgenic mice were backcrossed to C57BL/6J. Two lines were generated. RBRC02095 (commonly-used), RBRC03162.|
|Strain description||The TH-GFP transgenic mouse was generated to express GFP in the majority of midbrain dopamine neurons under the control of the rat TH gene promoter. Dopamine is a neurotransmitter which mediates motor control, cognition, emotion, reward, and neuroendocrine functions. Dysfunction of dopamine-producing neurons in the midbrain is involved in some neurological and neuropsychiatric diseases such as Parkinson's disease and schizophrenia. These dopamine neurons express tyrosine hydroxylase (TH), a key enzyme of catecholamine biosynthesis. This transgenic mouse is useful for visualizing dopamine neurons to study the physiology and pathogenesis of dopamine neurons. Homozygous transgenic mice are lethal.|
|Colony maintenance||Carrier x Noncarrier [C57BL/6JJcl]|
|Promoter||rat tyrosine hydroxylase (TH) promoter|
|Symbol name||Green Fluorescent Protein (Jellyfish)|
|Common name||No Data|
|Symbol description||No Data|
|References||J Neurochem. 2002 Jul;82(2):295-304.
Dynamics of tyrosine hydroxylase promoter activity during midbrain dopaminergic neuron development.
|Research applications||Fluorescent Proteins/lacZ System,
|Specific Term and Conditions||The following terms and conditions will be requested by the DEPOSITOR.
Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
J. Neurochem., 82, 295-304 (2002).
|2||Mouse of the Month May 2008
|3||GFP Transfer License (Japanese / English)
Please fill in the Schedule A, and submit two signed copies to us together with two signed copies of RIKEN BRC’s MTA. Please also read Schedule B.
Lab HP (Japanese)
|4||Genotyping protocol <PCR>|
|Depositor||Kobayashi, Kazuto (Fukushima Medical University)|
|Strain Status /
|Sensory Input Regulates Spatial and Subtype-Specific Patterns of Neuronal Turnover in the Adult Olfactory Bulb.||J Neurosci.31(32): 11587-11596 (2011).(21832189)|
|Sawada M, Kaneko N, Inada H, Wake H, Kato Y, Yanagawa Y, Kobayashi K, Nemoto T, Nabekura J, Sawamoto K.|
|Structural basis for serotonergic regulation of neural circuits in the mouse olfactory bulb.||J Comp Neurol. (2014).(25234191)|
|Suzuki Y, Kiyokage E, Sohn J, Hioki H, Toida K.|
|Identification of a Vav2-dependent mechanism for GDNF/Ret control of mesolimbic DAT trafficking.||Nat. Neurosci. (2015).(26147533)|
|Zhu S, Zhao C, Wu Y, Yang Q, Shao A, Wang T, Wu J, Yin Y, Li Y, Hou J, Zhang X, Zhou G, Gu X, Wang X, Bustelo XR, Zhou J.|
|Differential contribution of Ih to the integration of excitatory synaptic inputs in Substantia Nigra pars compacta and Ventral Tegmental Area dopaminergic neurons.||Eur. J. Neurosci. (2015).(26354486)|
|Masi A,, Narducci R, Resta F, Carbone C, Kobayashi K, Mannaioni G.|
|Spatial distribution of synapses on tyrosine hydroxylase expressing juxtaglomerular cells in the mouse olfactory glomerulus.||J. Comp. Neurol. (2016).(27864931)|
|Kiyokage E, Kobayashi K, Toida K.|