RBRC02096, B6.Cg-Tg(CAG-floxed Neo-EGFP)REP08Osb

RBRC No. RBRC02096
Type Transgene CongenicCartagena wks
Species Mus musculus
Strain name B6.Cg-Tg(CAG-floxed Neo-EGFP)REP08Osb
Former Common name REP-08
H-2 Haplotype No Data
Background strain C57BL/6
Appearance
1 Appearance black
Genotype a/a B/B C/C
Strain development Developed by Masato Ikawa, Faculty of Pharmaceutical Sciences, Osaka University in 2004. The transgene was injected into the D3 ES cells. The mutant mice were crossed to C57BL/6NCrSlc.
Strain description CAG-GFP conditional transgenic mice (REP08 mice). The transgene consists of the CAG promoter and the EGFP cDNA separated by a floxed neo cassette. When the floxed neo cassette is excised by crossing with Cre mice, EGFP is expressed. The transgene was injected into D3 ES cells to generate mice having a single copy of the transgene. The level of GFP fluorescence is relatively low (Developer, personal communication).
Colony maintenance Carrier x Carrier [C57BL/6NCrSlc]
Health Report
Gene Details
Promoter No Data
1 Symbol loxP
Symbol name phage P1 loxP
Chromosome UN
Common name No Data
Symbol description No Data
Promoter CAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA)
2 Symbol neo
Symbol name neomycin resistance gene (E. coli)
Chromosome UN
Common name neo; neomycin;
Symbol description No Data
3 Symbol GFP
Symbol name Green Fluorescent Protein (Jellyfish)
Chromosome UN
Common name No Data
Symbol description No Data
References Biochem Biophys Res Commun. 2004 Aug 20;321(2):275-9.

Lineage-specific cell disruption in living mice by Cre-mediated expression of diphtheria toxin A chain.
Research applications Cre/loxP system,
Fluorescent Proteins/lacZ System,
General Purpose
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
Biochem Biophys Res Commun., 321, 275-279 (2004).
RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or not-for-profit research is requested to enter into a Material Transfer Agreement with Osaka University (http://www.uic.osaka-u.ac.jp/en/index.html). The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the BIOLOGICAL RESOURCES. The RECIPIENT which wants to use the BIOLOGICAL RESOURCE even after five years must obtain a written consent from the DEPOSITOR again.
Additional information
1 GFP Transfer License (Japanese / English)
Please fill in the Schedule A, and submit two signed copies to us together with two signed copies of RIKEN BRC’s MTA. Please also read Schedule B.
Lab HP
2 Genotyping protocol <PCR>
Depositor Okabe, Masaru (Osaka university) Okabe, Masaru
Strain Status /
Availability
(Expected delivery)


Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months
BRC mice in Publications
Title Journal
(PMID)
Author
Hyaluronan deficiency in tumor stroma impairs macrophage trafficking and tumor neovascularization. Cancer Res.70(18): 7073-83 (2010).(20823158)
Kobayashi N, Miyoshi S, Mikami T, Koyama H, Kitazawa M, Takeoka M, Sano K, Amano J, Isogai Z, Niida S, Oguri K, Okayama M, McDonald JA, Kimata K, Taniguchi S, Itano N.
Wnt5a-Ror2 signaling between osteoblast-lineage cells and osteoclast precursors enhances osteoclastogenesis. Nat Med.18(3): 405-12 (2012).(22344299)
Maeda K, Kobayashi Y, Udagawa N, Uehara S, Ishihara A, Mizoguchi T, Kikuchi Y, Takada I, Kato S, Kani S, Nishita M, Marumo K, Martin TJ, Minami Y, Takahashi N.
Epithelial pten controls acute lung injury and fibrosis by regulating alveolar epithelial cell integrity. Am J Respir Crit Care Med.187(3): 262-75 (2013).(23239155)
Miyoshi K, Yanagi S, Kawahara K, Nishio M, Tsubouchi H, Imazu Y, Koshida R, Matsumoto N, Taguchi A, Yamashita S, Suzuki A, Nakazato M.