RBRC02140, B6.Cg-Tg(DBH-tauGFP)17

RBRC No. RBRC02140
Type Transgene CongenicCartagena
Species Mus musculus
Strain name B6.Cg-Tg(DBH-tauGFP)17
Former Common name B6.Cg-(DBH-tauGFP)17
H-2 Haplotype No Data
Background strain C57BL/6JJcl
Appearance
1 Appearance No Data
Genotype No Data
Strain development Developed by Kazuto Kobayashi, Fukushima Medical University School of Medicine in 1998. The transgene was injected into the pronuclei of B6D2F2 fertilized eggs. The Tg mice were backcrossed to C57BL/6J over 20 times. Line 17 (RBRC02140), line 36 (RBRC02141).
Strain description These transgenic mice express a fusion gene of bovine tau protein and GFP under the control of the human DBH promoter in typical noradrenergic and adrenergic cell groups. Line 17 and 36 were generated. The expression level of Tg in each line was following order: 17 > 36 (developer's information).
Health Report No Data
Gene Details
Promoter human dopamine beta hydroxylase (DBH) promoter
1 Symbol GFP
Symbol name Green Fluorescent Protein (Jellyfish)
Chromosome UN
Common name No Data
Symbol description No Data
2 Symbol tau
Symbol name bovine tau protein cDNA
Chromosome UN
Common name No Data
Symbol description No Data
References

Research applications Fluorescent Proteins/lacZ System
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
Neurogastroenterol. Motil. 22(3):331-e86. PMID: 19775251
Additional information
1 GFP Transfer License (Japanese / English)
Please fill in the Schedule A, and submit two signed copies to us together with two signed copies of RIKEN BRC’s MTA. Please also read Schedule B.
Lab HP (Japanese)
2 Genotyping protocol <PCR>
Depositor Kobayashi, Kazuto (Fukushima Medical University) Kobayashi, Kazuto
Strain Status /
Availability
(Expected delivery)


Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months