RBRC02142, B6.129S2-Pdcd1<tm1Hon>/HonRbrc

RBRC No. RBRC02142
Type Targeted Mutation CongenicCartagena wks
Species Mus musculus
Strain name B6.129S2-Pdcd1<tm1Hon>/HonRbrc
Former Common name C57BL/6-PD-1KO, C57BL/6-Pdcd1-/- mouse
H-2 Haplotype No Data
ES cell line D3 [129S2/SvPas]
Background strain C57BL/6
Appearance
1 Appearance black
Genotype (a/a B/B C/C)
Strain development Developed by Tasuku Honjo, Graduate School of Medicine, Kyoto University in 1998. The knockout construct was electoroporated into D3 ES cells derived from 129S2/SvPas. The mutant mice were backcrossed to C57BL/6 over 11 generations.
Strain description PD1 knockout mice. EcoRV site of exon 3 to PvuII site of exon 5 was replaced with noemycin selection cassette. PD1 (Pdcd1, programmed cell death 1) gene is an immunoinhibitory receptor that belongs to the CD28 family, plays a role in the negative control of proliferation, differentiation and class switching of B cells. PD1 deficiency has been shown to develop different forms of autoimmune diseases on different backgrounds of mice. Homozygous PD1 mutant mice of C57BL/6 background show moderate but consistent splenomegaly, and spontaneously develop characteristic lupus-like proliferative arthritis and golmerulonephritis with the age.
Colony maintenance Homozygote x Homozygote.
Health Report
Gene Details
Promoter No Data
1 Symbol Pdcd1
Symbol name programmed cell death 1
Chromosome 1
Common name Ly101, PD-1, Pdc1
Symbol description No Data
Promoter mouse phosphoglycerate kinase promoter (PGK promoter)
2 Symbol neo
Symbol name neomycin resistance gene (E. coli)
Chromosome 1
Common name neo; neomycin;
Symbol description No Data
References Immunity. 1999 Aug;11(2):141-51.
Int Immunol. 1998 Oct;10(10):1563-72.

Development of lupus-like autoimmune diseases by disruption of the PD-1 gene encoding an ITIM motif-carrying immunoreceptor.
Development of lupus-like autoimmune diseases by disruption of the PD-1 gene encoding an ITIM motif-carrying immunoreceptor.
Research applications Immunology and Inflammation Research
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
1) The RECIPIENT shall use the BIOLOGICAL RESOURCE only for academic research for the purpose of publishing the research results.
2) In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR and a citation of the following literature(s) designated by the DEPOSITOR are requested. Science 291, 319-322 (2001).
3) RECIPIENT shall notify the PROVIDER upon filing a patent application claiming modification of the BIOLOGICAL RESOURCE or method(s) of manufacture or use(s) of the BIOLOGICAL RESOURCE.
Additional information
1 Mouse of the Month Jul 2012
Genetic Background
Breeding characters
2 Honjo Lab HP
3 Genotyping protocol <PCR>
Depositor Honjo, Tasuku (Graduate School of Medicine, Kyoto University) Honjo, Tasuku
Strain Status /
Availability
(Expected delivery)


Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months

Live
Live mouse :
BRC mice in Publications
Title Journal
(PMID)
Author
Programmed death 1 signaling on chronic myeloid leukemia-specific T cells results in T-cell exhaustion and disease progression. Blood.114(8): 1528-36 (2009).(19420358)
Sabine Mumprecht, Christian Sch rch, Juerg Schwaller, Max Solenthaler, and Adrian F. Ochsenbein.
The inhibitory receptor PD-1 regulates IgA selection and bacterial composition in the gut. Science.336(6080): 485-9 (2012).(22539724)
Kawamoto S, Tran TH, Maruya M, Suzuki K, Doi Y, Tsutsui Y, Kato LM, Fagarasan S.
Programmed cell death 1 forms negative costimulatory microclusters that directly inhibit T cell receptor signaling by recruiting phosphatase SHP2. J Exp Med. (2012).(22641383)
Yokosuka T, Takamatsu M, Kobayashi-Imanishi W, Hashimoto-Tane A, Azuma M, Saito T.
PD-1 Dependent Exhaustion of CD8<sup>+</sup> T Cells Drives Chronic Malaria. Cell Rep. (2013).(24316071)
Horne-Debets JM, Faleiro R, Karunarathne DS, Liu XQ, Lineburg KE, Poh CM, Grotenbreg GM, Hill GR, Macdonald KP, Good MF, Renia L, Ahmed R, Sharpe AH, Wykes MN.
Metabolic shift induced by systemic activation of T cells in PD-1-deficient mice perturbs brain monoamines and emotional behavior. Nat. Immunol.18(12): 1342-1352 (2017).(29058703)
Miyajima M, Zhang B, Sugiura Y, Sonomura K, Guerrini MM, Tsutsui Y, Maruya M, Vogelzang A, Chamoto K, Honda K, Hikida T, Ito S, Qin H, Sanuki R, Suzuki K, Furukawa T, Ishihama Y, Matsuda F, Suematsu M, Honjo T, Fagarasan S.