RBRC02192, B6.129-Mapk14<tm1.2Otsu>

RBRC No. RBRC02192
Type Targeted MutationCartagena wks
Species Mus musculus
Strain name B6.129-Mapk14<tm1.2Otsu>
Former Common name p38α-flox mouse
H-2 Haplotype No Data
ES cell line R1 [(129X1/SvJ x 129S1/Sv)F1-Kitl<+>]
Background strain C57BL/6JJcl
1 Appearance black
Genotype a/a B/B C/C
Strain development Developed by Kinya Otsu, Osaka University Graduate School of Medicine in 2000. The targeting construct was electoroporated into R1 ES cells derived from (129X1/SvJ x 129S1/Sv)F1-Kitl<+>. The mutant mice were backcrossed to C57BL/6J.
Strain description Mitogen-activated protein (MAP) kinase cascades are highly conserved signal transduction pathways involved in a variety of physiological events. The MAP kinase family consists of extracellular signal-regulated protein kinase (ERK), c-Jun NH2-terminal protein kinase (JNK), and p38 MAP kinase. p38 alpha, one of the p38 MAP kinase subfamily, is widely expressed and has an important function in cytokine production and the response to many types of stress. Because p38alpha-deficient mice (BRC00361 C57BL/6-TgH(p38)/118) showed embryonic lethality at midgestation, this strain is useful for the research of p38 MAP kinase pathway in various tissues using Cre-loxP system.
Colony maintenance Homozygote x Homozygote [or Crossing to C57BL/6JJcl]. Homozygous mutant mice are viable and fertile.
Health Report
Gene Details
Promoter No Data
1 Symbol Mapk14
Symbol name mitogen activated protein kinase 14
Chromosome 17
Common name Crk1, Csbp1, CSBP2, Mxi2, p38, p38 MAP Kinase, p38-alpha, p38MAPK, tRNA synthetase cofactor p38
Symbol description No Data
2 Symbol loxP
Symbol name phage P1 loxP
Chromosome 17
Common name No Data
Symbol description No Data
References Mol Cell Biol 2004 Dec;24(24):10611-20.

p38alpha mitogen-activated protein kinase plays a critical role in cardiomyocyte survival but not in cardiac hypertrophic growth in response to pressure overload.
Research applications Cell Biology Research,
Cre/loxP system,
General Purpose
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
Mol. Cell. Biol., 24, 10611-10620 (2004).
In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
Additional information
1 Lab HP (Japanese)
2 Mouse of the Month Jul 2007
Genetic Background
3 Genotyping protocol <PCR>
Depositor Otsu, Kinya (King’s College London) Otsu, Kinya
Strain Status /
(Expected delivery)

Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months
BRC mice in Publications
Title Journal
Bidirectional regulation of neutrophil migration by mitogen-activated protein kinases. Nat Immunol.13(5): 457-64 (2012).(22447027)
Liu X, Ma B, Malik AB, Tang H, Yang T, Sun B, Wang G, Minshall RD, Li Y, Zhao Y, Ye RD, Xu J.
Mice with a Conditional Deletion of the Neurotrophin Receptor TrkB Are Dwarfed, and Are Similar to Mice with a MAPK14 Deletion. PLoS One.8(6): e66206 (2013).(23776632)
Hutchison MR
Sex-specific control of CNS autoimmunity by p38 MAPK signaling in myeloid cells. Ann Neurol. (2013).(24027119)
Krementsov DN, Noubade R, Dragon JA, Otsu K, Rincon M, Teuscher C.
Deficiency of Neuronal p38 MAPK Attenuates Amyloid Pathology in Alzheimer Disease Mouse and Cell Models through Facilitating Lysosomal Degradation of BACE1. J. Biol. Chem.291(5): 2067-2079 (2015).(26663083)
Schnöer L, Hao W, Qin Y, Liu S, Tomic I, Liu X, Fassbender K, Liu Y.