RBRC No. RBRC02228
Type TransgeneCartagena
Species Mus musculus
Strain name TOP-GAL
Former Common name No Data
H-2 Haplotype No Data
Donor strain (C57BL/6 x C3H)F2
Background strain ICR
1 Appearance albino
Genotype c/c
Strain development Developed by Yumiko Saga, National Institute of Genetics in 2005. The transgene was injected into the pronuclei of (C57BL/6 x C3H)F2 fertilized eggs. ICR mixed background.
Strain description Transgenic mice for visualizing Wnt signal pathway. Wnt proteins belong to a family of secreted and lipid-modified glycoprotein that can activate intracellular signaling pathways, such as the Wnt/planar cell polarity, the Wnt/calcium and the Wnt beta-catenin or so-called Wnt canonical signaling pathways. The TOP-GAL transgenic mice are a reporter line which can visualize the Wnt canonical pathway by beta-galactosidase activity. The transgenic mice were generated with ins-TOPGAL that accurately drive beta-galactosidase under TCF/beta-catenin. These transgenic mice will provide you unique opportunity to investigate the function of Wnt canonical pathway in vivo.
Colony maintenance Carrier x Noncarrier [or Crossing to Jcl:MCH(ICR)]. Tg mice are viable and fertile.
Health Report
Gene Details
Promoter No Data
1 Symbol nls
Symbol name SV40 large T antigen nuclear localization signal (NLS)
Chromosome UN
Common name No Data
Symbol description No Data
Promoter TOPFLUSH promoter
2 Symbol lacZ
Symbol name beta-galactosidase (E. coli)
Chromosome UN
Common name No Data
Symbol description E. coli beta galactosidase
References Genesis. 2007 Feb;45(2):90-100.

GFP transgenic mice reveal active canonical Wnt signal in neonatal brain and in adult liver and spleen.
Research applications Developmental Biology Research,
Fluorescent Proteins/lacZ System,
General Purpose
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
Genesis, 45, 90-100 (2007).
In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
Additional information
1 Saga Lab HP
2 Mouse of the Month Aug 2009
Genetic Background
3 Genotyping protocol <PCR>
Depositor Saga, Yumiko (National Institute of Genetics) Saga, Yumiko
Strain Status /
(Expected delivery)

Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months
BRC mice in Publications
Title Journal
Investigating Tonic Wnt Signaling Throughout the Adult CNS and in the Hippocampal Neurogenic Niche of BatGal and Ins-TopGal Mice. Cell Mol Neurobiol. (2012).(22491991)
Garbe DS, Ring RH.
beta-CateninC429S mice exhibit sterility consequent to spatiotemporally sustained Wnt signalling in the internal genitalia. Sci Rep.4: 6959 (2014).(25376241)
Murata T, Ishitsuka Y, Karouji K, Kaneda H, Toki H, Nakai Y, Makino S, Fukumura R, Kotaki H, Wakana S, Noda T, Gondo Y.
Differential requirements for beta-catenin in murine prostate cancer originating from basal versus luminal cells. J. Pathol.236(3): 290-301 (2015).(25712462)
Lu TL, Chen CM.
Bcl11b SWI/SNF-complex subunit modulates intestinal adenoma and regeneration after -irradiation through Wnt/ -catenin pathway. Carcinogenesis (2015).(25827435)
Sakamaki A, Katsuragi Y, Otsuka K, Tomita M, Obata M, Iwasaki T, Abe M, Sato T, Ochiai M, Sakuraba Y, Aoyagi Y, Gondo Y, Sakimura K, Nakagama H, Mishima Y, Kominami R.