RBRC02250, C57BL/6J-Tg(CAG-Cox8/EGFP)49Rin

RBRC No. RBRC02250
Type TransgeneCartagena wks
Species Mus musculus
Strain name C57BL/6J-Tg(CAG-Cox8/EGFP)49Rin
Former Common name mtGFP-Tg
H-2 Haplotype No Data
Background strain C57BL/6JJcl
Appearance
1 Appearance black
Genotype a/a B/B C/C
Strain development Developed by Hiroshi Shitara and Hiromichi Yonekawa, Tokyo Metropolitan Institute of Medical Science in 2001. C57BL/6J background.
Strain description These transgenic express GFP in mitochondoria. This strain is useful for morphological analysis of mitochondoria in vivo. Tg hemizygous mice have a tendency to exhibit small body size, and females of hemizygote show lower reproductive performance. Homozygous mice are infertile.
Colony maintenance Carrier x Noncarrier [C57BL/6JJcl]
Health Report
Gene Details
Promoter No Data
1 Symbol GFP
Symbol name Green Fluorescent Protein (Jellyfish)
Chromosome UN
Common name No Data
Symbol description No Data
2 Symbol HBB2
Symbol name rabbit beta-globin poly A
Chromosome UN
Common name No Data
Symbol description No Data
3 Symbol MLS
Symbol name mitochondria localization signal (mouse cytochrome C)
Chromosome UN
Common name No Data
Symbol description No Data
Promoter CAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA)
4 Symbol Cox8a
Symbol name cytochrome c oxidase, subunit VIIIa
Chromosome UN
Common name COX VIII-L, COX8L
Symbol description No Data
References

Research applications Cell Biology Research,
Fluorescent Proteins/lacZ System
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR/DEVELOPER.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
FEBS Letters, 500, 7-11 (2001). Exp. Anim., 59, 99-103 (2010).
In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR. The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use with the results from the use of the BIOLOGICAL RESOURCE.
Additional information
1 GFP Transfer License (Japanese / English)
Please fill in the Schedule A, and submit two signed copies to us together with two signed copies of RIKEN BRC’s MTA. Please also read Schedule B.
Organization HP
2 Genotyping protocol <PCR>
Depositor Yonekawa, Hiromichi (Tokyo Metropolitan Institute of Medical Science) Yonekawa, Hiromichi
Strain Status /
Availability
(Expected delivery)


Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months
BRC mice in Publications
Title Journal
(PMID)
Author
Mitochondrial physiology and gene expression analyses reveal metabolic and translational dysregulation in oocyte-induced somatic nuclear reprogramming. PLoS One.7(6): e36850 (2012).(22693623)
Esteves TC, Psathaki OE, Pfeiffer MJ, Balbach ST, Zeuschner D, Shitara H, Yonekawa H, Siatkowski M, Fuellen G, Boiani M.