RBRC02260, B6.129S-Trp53<tm2Mok>

RBRC No. RBRC02260
Type Targeted MutationCartagena wks
Species Mus musculus
Strain name B6.129S-Trp53<tm2Mok>
Former Common name p53KO, PFZ, PFZ632, B6-p53 KO
H-2 Haplotype No Data
ES cell line CCE/EK.CCE [129S/SvEv-Gpi1]
Background strain C57BL/6JJcl
Appearance
1 Appearance black
Genotype a/a B/B C/C
Strain development Developed by Motoya Katsuki, National Institutes of Natural Sciences (Medical Institute of Bioregulation, Kyushu University).
Strain description p53 (Trp53) gene knockout mice (lacZ reporter).
Colony maintenance Heterozygote x Wild-type [C57BL/6JJcl]
Health Report
Gene Details
Promoter No Data
1 Symbol Trp53
Symbol name transformation-related protein 53
Chromosome 11
Common name p53
Symbol description No Data
2 Symbol lacZ
Symbol name beta-galactosidase (E. coli)
Chromosome 11
Common name No Data
Symbol description E. coli beta galactosidase
References Biochem Biophys Res Commun. 1994 Jul 29;202(2):830-7.

Gene replacement of the p53 gene with the lacZ gene in mouse embryonic stem cells and mice by using two steps of homologous recombination.
Research applications Cancer Research,
Cell Biology Research,
Fluorescent Proteins/lacZ System,
Immunology and Inflammation Research,
Mouse Models for Human Disease
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
Biochem. Biophys. Res. Commun., 202, 830-837 (1994).
In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to Dr. Motoya Katsuki is requested. The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
Additional information
1 No Data
2 Genotyping protocol <PCR>
Depositor Sasaoka, Toshikuni (Niigata university) Sasaoka, Toshikuni
Strain Status /
Availability
(Expected delivery)


Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months