RBRC02290, STOCK Tg(Nanog-GFP,Puro)1Yam

RBRC No. RBRC02290
Type TransgeneCartagena wks
Species Mus musculus
Strain name STOCK Tg(Nanog-GFP,Puro)1Yam
Former Common name No Data
H-2 Haplotype No Data
ES cell line RF8 [129S4/SvJae]
Background strain No Data
1 Appearance No Data
Genotype No Data
Strain development Developed by Dr. Shinya Yamanaka, Institute for Frontier Medical Sciences, Kyoto University in 2003. The construct was injected into RF8 ES cells derived from 129S4/SvJae. 129, C57BL/6 and DBA/2 mixed background.
Strain description Nanog-GFP transgenic mice. Nanog gene is a good candidate marker for maintaining pluripotent differentiation, because of the loss of pluripotent epiblast in Nanog KO mice, the tendency of Nanog-null ES cells to differenciate spontaneously, and the capability of Nanog-overexpressed ES cells to maintain self-renewal independently of leukaemia inhibitory factor (LIF). This Nanog-GFP transgene was generated by insertion of GFP-IRES-puromycin resistance gene (Puror) cassette into the 5′ untranslated region of a bacterial artificial chromosome (BAC) containing the mouse Nanog gene. ES cells with the Nanog-GFP transgene were positive for GFP, but became negative when differentiation was induced. Using this system, pluripotent stem cells competent for germline chimaeras can be induced from Nanog-GFP transgenic mouse fibroblasts by introduction of candidate genes for pluripotency. Tg homozygous and hemizygous mice were viable and fertile. Male-biased sex ratios was observed (199 females : 261 males)(details unknown).
Colony maintenance Carrier x Carrier (Homozygote x Homozygote)
Health Report
Gene Details
Promoter No Data
1 Symbol IRES
Symbol name internal ribosomal entry site (EMCV)
Chromosome UN
Common name internal ribosomal entry site from EMCV
Symbol description No Data
2 Symbol Frt
Symbol name yeast FRT (flippase recombination target) site
Chromosome UN
Common name No Data
Symbol description No Data
3 Symbol GH
Symbol name Growth hormone polyA (Bovine)
Chromosome UN
Common name No Data
Symbol description No Data
4 Symbol puro
Symbol name puromycin resistance gene (Streptomyces alboniger)
Chromosome UN
Common name No Data
Symbol description No Data
Promoter Nanog promoter
5 Symbol GFP
Symbol name Green Fluorescent Protein (Jellyfish)
Chromosome UN
Common name No Data
Symbol description No Data
References Nature. 2007 Jul 19;448(7151):313-7. Epub 2007 Jun 6.

Generation of germline-competent induced pluripotent stem cells.
Research applications Cell Biology Research,
Developmental Biology Research,
FLP/frt system,
Fluorescent Proteins/lacZ System
Specific Term and Conditions For inquiries please mail us at animal@brc.riken.jp.
Additional information
1 Mouse of the Month Dec 2007
Mouse of the Month Oct 2012
2 Distribution of the iPS cells (Japanese/ English)
GFP Transfer License (Japanese / English)
Please fill in the Schedule A, and submit two signed copies to us together with two signed copies of RIKEN BRC’s MTA. Please also read Schedule B.
3 Genotyping protocol <PCR>
Depositor Yamanaka, Shinya (Center for iPS Cell Research and Application, Kyoto University) Yamanaka, Shinya
Strain Status /
(Expected delivery)

Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months

Live mouse :
BRC mice in Publications
Title Journal
Conversion of ancestral fibroblasts to induced pluripotent stem cells. Stem Cells.28(2): 213-20 (2010).(20020427)
Araki R, Jincho Y, Hoki Y, Nakamura M, Tamura C, Ando S, Kasama Y, Abe M.
Generation of mouse-induced pluripotent stem cells with plasmid vectors. Nat Protoc.5(3): 418-28 (2010).(20203661)
Okita K, Hong H, Takahashi K, Yamanaka S.
Generation of Genome Integration-free Induced Pluripotent Stem Cells from Fibroblasts of C57BL/6 Mice without c-Myc Transduction. J Biol Chem.285(34): 26384-9 (2010).(20554535)
Jincho Y, Araki R, Hoki Y, Tamura C, Nakamura M, Ando S, Kasama Y, Abe M.
Efficient generation of functional dopaminergic neurons from human induced pluripotent stem cells under defined conditions. Stem Cells.28(10): 1893-904 (2010).(20715183)
Swistowski A, Peng J, Liu Q, Mali P, Rao MS, Cheng L, Zeng X.
A germ cell-specific gene, Prmt5, works in somatic cell reprogramming. J Biol Chem.286(12): 10641-8 (2011).(21270127)
Nagamatsu G, Kosaka T, Kawasumi M, Kinoshita T, Takubo K, Akiyama H, Sudo T, Kobayashi T, Oya M, Suda T.
Crucial Role of c-Myc in the Generation of Induced Pluripotent Stem Cells. Stem Cells. (2011).(21732496)
Araki R, Hoki Y, Uda M, Nakamura M, Jincho Y, Tamura C, Sunayama M, Ando S, Sugiura M, Yoshida MA, Kasama Y, Abe M.
Tracing the Conversion Process for Primordial Germ Cells to Pluripotent Stem Cells in Mice. Biol Reprod. (2012).(22423052)
Nagamatsu G, Kosaka T, Saito S, Takubo K, Akiyama H, Sudo T, Horimoto K, Oya M, Suda T.
Role of Lefty in the anti tumor activity of human adult liver stem cells. Oncogene.32(7): 819-26 (2013).(22469982)
Cavallari C, Fonsato V, Herrera MB, Bruno S, Tetta C, Camussi G.
Foxd1 is a mediator and indicator of the cell reprogramming process. Nat Commun.5: 3197 (2014).(24496101)
Koga M, Matsuda M, Kawamura T, Sogo T, Shigeno A, Nishida E, Ebisuya M.
Histone variants enriched in oocytes enhance reprogramming to induced pluripotent stem cells. Cell Stem Cell.14(2): 217-27 (2014).(24506885)
Shinagawa T, Takagi T, Tsukamoto D, Tomaru C, Huynh LM, Sivaraman P, Kumarevel T, Inoue K, Nakato R, Katou Y, Sado T, Takahashi S, Ogura A, Shirahige K, Ishii S.
Preferential gene expression and epigenetic memory of induced pluripotent stem cells derived from mouse pancreas. Genes Cells20(5): 367-81 (2015).(25727848)
Nukaya D, Minami K, Hoshikawa R, Yokoi N, Seino S.
Combined Overexpression of JARID2, PRDM14, ESRRB, and SALL4A Dramatically Improves Efficiency and Kinetics of Reprogramming to Induced Pluripotent Stem Cells. Stem Cells (2015).(26523946)
Iseki H, Nakachi Y, Hishida T, Yamashita-Sugahara Y, Hirasaki M, Ueda A, Tanimoto Y, Iijima S, Sugiyama F, Yagami KI, Takahashi S, Okuda A, Okazaki Y.