RBRC02298, C.Cg-Il3<tm1Glli>

RBRC No. RBRC02298
Type Targeted MutationCartagena
Species Mus musculus
Strain name C.Cg-Il3<tm1Glli>
Former Common name IL-3 knock-out mouse
H-2 Haplotype No Data
ES cell line D3 [129S2/SvPas]
Background strain BALB/cAnNTac
Appearance
1 Appearance albino
Genotype A/A b/b c/c
Strain development Developed by Dr. Glenn Dranoff, Dana-Farber Cancer Institute, Harvard Medical School in 1998. Mutant mice were backcrossed to C57BL/6 for 9 gnerations, and then to BALB/cAnNTac for 3 generations. Deposited by Dr. Shigeo Koyasu, Keio University School of Medicine.
Strain description Interluekin-3 is a growth factor of hematopoieic progenitors that yields multipotential blast cells, mast cells, basophils,neutrophils, macrophages, eosinophils, erythrocytes, megakaryocytes, and dendritic cells. IL-3 null mutant mice show unimpaired hematopoiesis, but contact hypersensitivity reactions are compromised.
Colony maintenance Backcross to BALB/c (Heterozygote x BALB/cAnNTac)
Health Report
Gene Details
Promoter No Data
1 Symbol Il3
Symbol name Interleukin 3
Chromosome 11
Common name BPA, Csfmu, HCGF, Il-3, MCGF, PSF
Symbol description No Data
2 Symbol poly A
Symbol name mouse PGK poly A
Chromosome 11
Common name No Data
Symbol description No Data
Promoter mouse phosphoglycerate kinase promoter (PGK promoter)
3 Symbol neo
Symbol name neomycin resistance gene (E. coli)
Chromosome 11
Common name neo; neomycin;
Symbol description No Data
References

Research applications Immunology and Inflammation Research
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR.
The RECIPIENT of BIOLOGICAL RESOURCE must obtain a prior written consent on use of it from the DEPOSITOR/DEVELOER (Glenn Dranoff, MD Harvard Medical School, Dana-Farber Cancer Institute E-mail: glenn_dranoff@dfci.harvard.edu).
Additional information
1 Lab HP
2 Genotyping protocol <PCR>
Depositor Koyasu, Shigeo (Keio University) Koyasu, Shigeo
Strain Status /
Availability
(Expected delivery)


Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months
BRC mice in Publications
Title Journal
(PMID)
Author
Skin thymic stromal lymphopoietin initiates Th2 responses through an orchestrated immune cascade. Nat Commun.4: 2847 (2013).(24284909)
Leyva-Castillo JM, Hener P, Michea P, Karasuyama H, Chan S, Soumelis V, Li M.
Basophil-derived interleukin-4 controls the function of natural helper cells, a member of ILC2s, in lung inflammation. Immunity.40(5): 758-71 (2014).(24837103)
Motomura Y, Morita H, Moro K, Nakae S, Artis D, Endo TA, Kuroki Y, Ohara O, Koyasu S, Kubo M.
Interleukin-3 amplifies acute inflammation and is a potential therapeutic target in sepsis. Science.347(6227): 1260-5. (2015).(25766237)
Weber GF, Chousterman BG, He S, Fenn AM, Nairz M, Anzai A, Brenner T, Uhle F, Iwamoto Y, Robbins CS, Noiret L, Maier SL, Zönchen T, Rahbari NN, Schöch S, Klotzsche-von Ameln A, Chavakis T, Weitz J, Hofer S, Weigand MA, Nahrendorf M, Weissleder R, Swirski FK.