RBRC02302, B6.129P2-Lyzs<tm1(cre)Ifo>

RBRC No. RBRC02302
Type Targeted MutationCartagena wks
Species Mus musculus
Strain name B6.129P2-Lyzs<tm1(cre)Ifo>
Former Common name LysM-Cre knock-in mice (C57BL/6)
H-2 Haplotype No Data
ES cell line E14 [129P2/OlaHsd]
Background strain C57BL/6NTac
Appearance
1 Appearance black
Genotype a/a B/B C/C
Strain development Developed by Technical University of Munich, Irmgard Foerster. Deposited by Shigeo Koyasu, School of Medicine, Keio University through Akita University Graduate School of Medicine. E14 ES cells were used to generate the knock-in mice. The mice were crossed to C57BL/6 for several times, and then backcrossed to C57BL/6NTac and BALB/cAnN, respectively (RBRC02302 and RBRC02303).
Strain description Lyzs (Lyz2, lysozyme 2) Cre knock-in mice, known as the LysMcre mouse. A NLS-cre cDNA was inserted into the endogenous ATG start site of Lyz2 gene. LysMcre mice allow for both specific and highly efficient Cre-mediated deletion of loxP-flanked target genes in myeloid cells. C57BL/6 background (RBRC02302) and BALB/c background (RBRC02303).
Colony maintenance Homozygote x Homozygote [or Crossing to C57BL/6NTac]
Health Report
Gene Details
Promoter No Data
1 Symbol Lyzs
Symbol name lysozyme
Chromosome 10
Common name LC, Lys-cre, LysM Cre, lysM/cre, LysMCre, LysMcre
Symbol description No Data
2 Symbol Frt
Symbol name yeast FRT (flippase recombination target) site
Chromosome 10
Common name No Data
Symbol description No Data
3 Symbol cre
Symbol name Phage P1 Cre recombinase
Chromosome 10
Common name No Data
Symbol description No Data
4 Symbol nls
Symbol name SV40 large T antigen nuclear localization signal (NLS)
Chromosome 10
Common name No Data
Symbol description No Data
References

Research applications Cell Biology Research,
Cre/loxP system,
FLP/frt system
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR.
The RECIPIENT of BIOLOGICAL RESOURCE must obtain a prior written consent on use of it from the DEVELOPER, Dr. Irmgard Foerster, Life and Medical Sciences (LIMES) Institute, University of Bonn E-mail: irmgard.foerster@uni-bonn.de.
Additional information
1 Lab HP
2 Genetic Background
3 Genotyping protocol <PCR>
Depositor Koyasu, Shigeo (Keio University) Koyasu, Shigeo
Strain Status /
Availability
(Expected delivery)


Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months

Live
Live mouse :
BRC mice in Publications
Title Journal
(PMID)
Author
IRF4 in Dendritic Cells Inhibits IL-12 Production and Controls Th1 Immune Responses against Leishmania major. J Immunol.192(5): 134288 (2014).(24489086)
Akbari M, Honma K, Kimura D, Miyakoda M, Kimura K, Matsuyama T, Yui K.
Prostaglandin Transporter OATP2A1/SLCO2A1 Is Essential for Body Temperature Regulation during Fever. J. Neurosci.38(24): 5584-5595 (2018).(29899035)
Nakamura Y, Nakanishi T, Shimada H, Shimizu J, Aotani R, Maruyama S, Higuchi K, Okura T, Deguchi Y, Tamai I.