RBRC02322, B6.129P2-Cmah<tm1Ykoz>

RBRC No. RBRC02322
Type Targeted MutationCartagena wks
Species Mus musculus
Strain name B6.129P2-Cmah<tm1Ykoz>
Former Common name Cmah null mouse, KZO
H-2 Haplotype No Data
ES cell line E14 [129P2/OlaHsd]
Background strain C57BL/6JJcl
Appearance
1 Appearance black
Genotype a/a B/B C/C
Strain development Developed by Hiromu Takematsu and Yasunori Kozutumi, Graduate School of Biostudies, Kyoto University in 2000. E14 ES cells were used. The mutant mice were backcrossed to C57BL/6J.
Strain description Cmah gene knockout mice. A neo cassette was inerted into the second coding exon. This mouse is deficient for the biosynthesis of N-glycolylneuramic acid, one of the major species of sialic acid. Due to the deficiency, sialic acid species of this mouse is almost 100% N-acetylneuraminic acid, which is the precursor of N-glycolylneuraminic acid in the biosynthetic pathway. Since CMAH is one of rare gene inactivated in human but retained in the other homonids such as chimpanzee. Thus, this mouse could by utilized to mimic human in terms of the sialic acid species expression.
Colony maintenance Heterozygote x Wild-type [C57BL/6JJcl]
Health Report
Gene Details
Promoter No Data
1 Symbol Cmah
Symbol name cytidine monophospho-N-acetylneuraminic acid hydroxylase
Chromosome 13
Common name CMP-NeuAc hydroxylase
Symbol description No Data
2 Symbol neo
Symbol name neomycin resistance gene (E. coli)
Chromosome 13
Common name neo; neomycin;
Symbol description No Data
References Mol Cell Biol. 2007 Feb 12;.

Germinal center marker GL7 probes activation-dependent repression of N-glycolylneuraminic acid, a sialic acid species involved in the negative modulation of B cell activation.
Research applications Metabolism Research
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
Mol. Cell. Biol., 27, 3008-3022 (2007).
In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
The RECIPIENT agrees to use of the BIOLOGICAL RESOURCE only for publication of research papers.
Additional information
1 No Data
2 Genotyping protocol <PCR>
Depositor Kozutsumi, Yasunori (Kyoto University) Kozutsumi, Yasunori
Strain Status /
Availability
(Expected delivery)


Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months