RBRC02332, B6.Cg-Tg(Drd2-YFP)364-5

RBRC No. RBRC02332
Type Transgene CongenicCartagena
Species Mus musculus
Strain name B6.Cg-Tg(Drd2-YFP)364-5
Former Common name No Data
H-2 Haplotype No Data
Background strain C57BL/6JJcl
Appearance
1 Appearance black
Genotype a/a B/B C/C
Strain development Developed by Dr. Kazuto Kobayashi, Fukushima Medical University School of Medicine in 2007. The Drd2-YFP transgene was injected into the pronuclei of C57BL/6J fertilized eggs.
Strain description This transgenic strain expresses YFP under the control of rat dopamine receptor 2 gene promoter.
Colony maintenance Hemizygote x Non carrier [C57BL/6JJcl]
Health Report
Gene Details
Promoter No Data
1 Symbol poly A
Symbol name SV40 poly A
Chromosome UN
Common name No Data
Symbol description No Data
Promoter rat Drd2 promoter
2 Symbol YFP
Symbol name Yellow Fluorescent Protein (Jellyfish)
Chromosome UN
Common name No Data
Symbol description No Data
References No Data

Research applications Fluorescent Proteins/lacZ System,
Neurobiology Research
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR.
Neuron, 89 (3) 550-565 (2016).
In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
The RECIPIENT shall contact the DEPOSITOR (Dr. Kazuto Kobayasi, kazuto@fmu.ac.jp) about the use of the BIOLOGICAL RESOURCE in advance.
Additional information
1 GFP Transfer License (Japanese / English)
Please fill in the Schedule A, and submit two signed copies to us together with two signed copies of RIKEN BRC’s MTA. Please also read Schedule B.
Lab HP (Japanese)
2 Genotyping protocol <PCR>
Depositor Kobayashi, Kazuto (Fukushima Medical University) Kobayashi, Kazuto
Strain Status /
Availability
(Expected delivery)


Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months
BRC mice in Publications
Title Journal
(PMID)
Author
Phosphoproteomics of the Dopamine Pathway Enables Discovery of Rap1 Activation as a Reward Signal In Vivo. Neuron89(3): 550-65. (2016).(26804993)
Nagai T, Nakamuta S, Kuroda K, Nakauchi S, Nishioka T, Takano T, Zhang X, Tsuboi D, Funahashi Y, Nakano T, Yoshimoto J, Kobayashi K, Uchigashima M, Watanabe M, Miura M, Nishi A, Kobayashi K, Yamada K, Amano M, Kaibuchi K.
Cell type-specific activation of mitogen-activated protein kinase in D1 receptor-expressing neurons of the nucleus accumbens potentiates stimulus-reward learning in mice. Sci. Rep.8(1): 14413. (2018).(30258218)
Bin Saifullah MA, Nagai T, Kuroda K, Wulaer B, Nabeshima T, Kaibuchi K, Yamada K.