RBRC02370, B6;129-Grn<tm1>

RBRC No. RBRC02370
Type Targeted MutationCartagena wks
Species Mus musculus
Strain name B6;129-Grn<tm1>
Former Common name Progranulin knock-out mouse
H-2 Haplotype No Data
ES cell line E14.1 [129P2/OlaHsd]
Background strain C57BL/6
1 Appearance black
Genotype a/a B/B C/C
Strain development Developed by Masugi Nishihara, Veterinary Medical Science, The University of Tokyo and Yoichiro Iwakura, Institute of Medical Science, The University of Tokyo in 2001. Targeting construct was electoroporated into E14.1 ES cells derived from 129P2/OlaHsd.
Strain description Grn, granulin knockout mice. A 4.7 kb fragment encoding exon 2-13 of Grn gene was replaced with a PGK-neo-bpA cassette. Homozygous mutant mice show a decrease in ejaculation incidence, enhanced aggressiveness and an elevated level of anxiety in male.
Colony maintenance Heterozygote x Wild-type [C57BL/6JJmsSlc].
Health Report
Gene Details
Promoter No Data
1 Symbol Grn
Symbol name granulin
Chromosome 11
Common name acrogranulin, epithelin, PC cell-derived growth factor, progranulin
Symbol description No Data
2 Symbol GH
Symbol name Growth hormone polyA (Bovine)
Chromosome 11
Common name No Data
Symbol description No Data
Promoter mouse phosphoglycerate kinase promoter (PGK promoter)
3 Symbol neo
Symbol name neomycin resistance gene (E. coli)
Chromosome 11
Common name neo; neomycin;
Symbol description No Data
References Behav Brain Res. 2007 Jul 20;.

Alteration of behavioural phenotype in mice by targeted disruption of the progranulin gene.
Research applications Neurobiology Research
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
Behav. Brain. Res., 185, 110-118 (2007).
The RECIPIENT of BIOLOGICAL RESOURCE must obtain a prior written consent on use of it from the DEPOSITOR/DEVELOPER.
Additional information
1 Genetic Background
2 Genotyping protocol <PCR>
Depositor Nishihara, Masugi (The University of Tokyo) Nishihara, Masugi
Strain Status /
(Expected delivery)

Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months
BRC mice in Publications
Title Journal
Deletion of progranulin exacerbates atherosclerosis in ApoE knockout mice. Cardiovasc Res.100(1): 125-33 (2013).(23847387)
Kawase R, Ohama T, Matsuyama A, Matsuwaki T, Okada T, Yamashita T, Yuasa-Kawase M, Nakaoka H, Nakatani K, Inagaki M, Tsubakio-Yamamoto K, Masuda D, Nakagawa-Toyama Y, Nishida M, Ohmoto Y, Nishihara M, Komuro I, Yamashita S.
Progressive retinal degeneration and accumulation of autofluorescent lipopigments in Progranulin deficient mice. Brain Res. (2014).(25234724)
Hafler BP, Klein ZA, Jimmy Zhou Z, Strittmatter SM.
Progranulin reduction is associated with increased tau phosphorylation in P301L tau transgenic mice. J. Neuropathol. Exp. Neurol.74(2): 158-65 (2015).(25575133)
Hosokawa M, Arai T, Masuda-Suzukake M, Kondo H, Matsuwaki T, Nishihara M, Hasegawa M, Akiyama H.
Progranulin plays crucial roles in preserving bone mass by inhibiting TNF- -induced osteoclastogenesis and promoting osteoblastic differentiation in mice. Biochem. Biophys. Res. Commun. (2015).(26297947)
Noguchi T, Ebina K, Hirao M, Kawase R, Ohama T, Yamashita S, Morimoto T, Koizumi K, Kitaguchi K, Matsuoka H, Kaneshiro S, Yoshikawa H.
Progranulin promotes the retinal precursor cell proliferation and the photoreceptor differentiation in the mouse retina. Sci. Rep. (2016).(27030285)
Kuse Y, Tsuruma K, Sugitani S, Izawa H, Ohno Y, Shimazawa M, Hara H.
Microglia increases the proliferation of retinal precursor cells during postnatal development. Mol. Vis.24: 536-545. (2018).(30090016)
Kuse Y, Ohuchi K, Nakamura S, Hara H, Shimazawa M.