RBRC02396, B6.129X1-Pknox1<tm1.1Nuk>

RBRC No. RBRC02396
Type Targeted MutationCartagena wks
Species Mus musculus
Strain name B6.129X1-Pknox1<tm1.1Nuk>
Former Common name Prep1LacZKIdeltaNeo, C57BL/6
H-2 Haplotype No Data
ES cell line R1 [(129X1/SvJ x 129S1/Sv)F1-Kitl<+>]
Background strain No Data
Appearance
1 Appearance black
Genotype a/a B/B C/C
Strain development Developed by Dr. Atsushi Kuroiwa, Nagoya University.
Strain description Prep1 (Pknox1) knockout mice. Prep1 gene is a member of the TALE class of homeodomain proteins and essential in embryonic development. Homozygous mutant mice are embryonic lethal (E9.5). This strain is useful for elucidation of the function of Prep1 gene during embryonic development.
Colony maintenance Backcross to C57BL/6 (Heterozygote x C57BL/6CrSlc)
Health Report
Gene Details
Promoter No Data
1 Symbol Pknox1
Symbol name Pbx/knotted 1 homeobox
Chromosome 17
Common name D17Wsu76e, PREP1
Symbol description No Data
2 Symbol lacZ
Symbol name beta-galactosidase (E. coli)
Chromosome 17
Common name No Data
Symbol description E. coli beta galactosidase
3 Symbol loxP
Symbol name phage P1 loxP
Chromosome 17
Common name No Data
Symbol description No Data
4 Symbol poly A
Symbol name SV40 poly A
Chromosome 17
Common name No Data
Symbol description No Data
References No Data

Research applications Cre/loxP system,
Fluorescent Proteins/lacZ System
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR.
In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for publication of research papers. The RECIPIENT of BIOLOGICAL RESOURCE must obtain a prior written consent on use of it from the DEPOSITOR/DEVELOPER. The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use with the results from the use of the BIOLOGICAL RESOURCE.
Additional information
1 Lab HP
2 Genotyping protocol <PCR>
Depositor Kuroiwa, Atsushi (Graduate School of Science, Nagoya University) Kuroiwa, Atsushi
Strain Status /
Availability
(Expected delivery)


Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months