RBRC02405, B6.Cg-4930583H14Rik<tm1Hmd>

RBRC No. RBRC02405
Type Targeted Mutation CongenicCartagena wks
Species Mus musculus
Strain name B6.Cg-4930583H14Rik<tm1Hmd>
Former Common name 4930583H14Rik flox
H-2 Haplotype No Data
ES cell line G4 [(129S6/SvEvTac x C57BL/6NCr)F1]
Background strain No Data
Appearance
1 Appearance No Data
Genotype No Data
Strain development Developed by Takako Maeda and Hiroshi Hamada, Institute for Molecular and Cellular Biology, Osaka University in 2007. G4 ES cells derived from (129S6 x C57BL/6)F1 were used. C57BL/6 and 129 mixed background.
Strain description 4930583H14Rik floxed mice. ORF region of the exon 4 was flanked by loxP sites.
Health Report
Gene Details
Promoter No Data
1 Symbol IRES
Symbol name internal ribosomal entry site (EMCV)
Chromosome 3
Common name internal ribosomal entry site from EMCV
Symbol description No Data
2 Symbol 4930583H14Rik
Symbol name RIKEN cDNA 4930583H14 gene
Chromosome 3
Common name No Data
Symbol description No Data
3 Symbol Frt
Symbol name yeast FRT (flippase recombination target) site
Chromosome 3
Common name No Data
Symbol description No Data
4 Symbol Myc-tag
Symbol name myc-epitope tag
Chromosome 3
Common name No Data
Symbol description No Data
5 Symbol lacZ
Symbol name beta-galactosidase (E. coli)
Chromosome 3
Common name No Data
Symbol description E. coli beta galactosidase
6 Symbol loxP
Symbol name phage P1 loxP
Chromosome 3
Common name No Data
Symbol description No Data
Promoter mouse phosphoglycerate kinase promoter (PGK promoter)
7 Symbol neo
Symbol name neomycin resistance gene (E. coli)
Chromosome 3
Common name neo; neomycin;
Symbol description No Data
References No Data

Research applications Cre/loxP system,
Fluorescent Proteins/lacZ System
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR.
The BIOLOGICAL RESOURCE must obtain a prior written consent on use of it from the DEPOSITOR/DEVELOPER.
Additional information
1 No Data
2 Genotyping protocol <PCR>
Depositor Hamada, Hiroshi (RIKEN Center for Developmental Biology) Hamada, Hiroshi
Strain Status /
Availability
(Expected delivery)


Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months