RBRC02415, C57BL/6-Tg(TRE-GFP)392Yasu

RBRC No. RBRC02415
Type TransgeneCartagena wks
Species Mus musculus
Strain name C57BL/6-Tg(TRE-GFP)392Yasu
Former Common name β-catenin/Tcf reporter mice
H-2 Haplotype No Data
Background strain C57BL/6JJcl
Appearance
1 Appearance No Data
Genotype No Data
Strain development Developed by Yasuhiro Yamada, Gifu University Graduate School of Medicine in 2007. The transgene was injected into the pronuclei of C57BL/6 fertilized eggs. Line 392 (main line) (RBRC02415), line 622 (rbrc02416) and line 628 (RBRC02417).
Strain description Beta-catenin/Tcf reporter mice. These mice express GFP gene under the control of the Tcf/Lef binding sites and a minimal thymidine kinase promoter from the TOPFLASH plasmid. Immunohistochemistry with a GFP antibody can be used to assess the Tg expression in the intestine. In other organs except for the intestine. In the splenocytes, expression of GFP was observed by FACS analysis, In other organs, the GFP expression patterns are not examined yet.
Health Report No Data
Gene Details
Promoter No Data
1 Symbol No Data
Symbol name SV40 polyA
Chromosome No Data
Common name No Data
Symbol description No Data
Promoter Tcf binding element, herpes simplex virus thymidine kinase promoter (HSV tk promoter)
2 Symbol GFP
Symbol name Green Fluorescent Protein (Jellyfish)
Chromosome UN
Common name No Data
Symbol description No Data
References Cancer Res. 2007 May 1;67(9):4079-87.
Carcinogenesis. 2008 Mar;29(3):666-72. Epub 2008 Jan 19.

Development of gastric tumors in Apc(Min/+) mice by the activation of the beta-catenin/Tcf signaling pathway.
Development of gastric tumors in Apc(Min/+) mice by the activation of the beta-catenin/Tcf signaling pathway.
Research applications Cancer Research,
Cell Biology Research,
Developmental Biology Research,
Fluorescent Proteins/lacZ System,
General Purpose,
Tet system
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR.
The RECIPIENT of BIOLOGICAL RESOURCE must obtain a prior written consent on use of it from the DEPOSITOR/DEVELOPER. The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for publication of research papers.
Additional information
1 GFP Transfer License (Japanese / English)
Please fill in the Schedule A, and submit two signed copies to us together with two signed copies of RIKEN BRC’s MTA. Please also read Schedule B.
Depositor Yamada, Yasuhiro (Gifu University, Graduate School of Medicine) Yamada, Yasuhiro
Strain Status /
Availability
(Expected delivery)


Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months