RBRC02565, ICR.Cg-Nanos3<tm2(cre)Ysa>/35YsaRbrc

RBRC No. RBRC02565
Type Targeted MutationCartagena wks
Species Mus musculus
Strain name ICR.Cg-Nanos3<tm2(cre)Ysa>/35YsaRbrc
Former Common name Nanos3-CrepA(neo)#35
H-2 Haplotype No Data
ES cell line TT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1]
Background strain No Data
Appearance
1 Appearance No Data
Genotype No Data
Strain development Developed by Dr. Yumiko Saga (2007).
Strain description These knock-in mice have a cre gene at the Nanos3 locus. Expression is detected in the early PGC and testis. Homozygous mutant mice are infertile.
Colony maintenance Sibling Mating (Hemizygote x Wild-type)
Health Report
Gene Details
Promoter No Data
1 Symbol Nanos3
Symbol name nanos homolog 3 (Drosophila)
Chromosome 8
Common name No Data
Symbol description No Data
2 Symbol Frt
Symbol name yeast FRT (flippase recombination target) site
Chromosome 8
Common name No Data
Symbol description No Data
3 Symbol Frt
Symbol name yeast FRT (flippase recombination target) site
Chromosome 8
Common name No Data
Symbol description No Data
4 Symbol cre
Symbol name Phage P1 Cre recombinase
Chromosome 8
Common name No Data
Symbol description No Data
Promoter mouse phosphoglycerate kinase promoter (PGK promoter)
5 Symbol neo
Symbol name neomycin resistance gene (E. coli)
Chromosome 8
Common name neo; neomycin;
Symbol description No Data
References Dev Biol. 2008 Jun 1;318(1):133-42. Epub 2008 Mar 20.

Nanos3 maintains the germ cell lineage in the mouse by suppressing both Bax-dependent and -independent apoptotic pathways.
Research applications Cre/loxP system,
Developmental Biology Research,
FLP/frt system
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
Dev. Biol., 318, 133-142 (2008).
Within a period of two years after deposition of the BIOLOGICAL RESOURCE, the RECIPIENT shall obtain a prior written consent on use of it from the DEPOSITOR. The RECIPIENT must contact the DEPOSITOR and notify him/her a content of the research. If the DEPOSITOR judges that the research should be a collaborative work, then the RECIPIENT must include the DEPOSITOR and/or the DEVELOPER’s names as co-authors.
Additional information
1 Saga Lab HP
2 Genotyping protocol <PCR>
Depositor Saga, Yumiko (National Institute of Genetics) Saga, Yumiko
Strain Status /
Availability
(Expected delivery)


Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months