RBRC02654, B6;129-Xist<tm4Sado>

RBRC No. RBRC02654
Type Targeted Mutation CongenicCartagena wks
Species Mus musculus
Strain name B6;129-Xist<tm4Sado>
Former Common name Xist; B6.Cg-Xist
H-2 Haplotype No Data
ES cell line R1 [(129X1/SvJ x 129S1/Sv)F1-Kitl<+>]
Background strain No Data
Appearance
1 Appearance No Data
Genotype No Data
Strain development Developed by Takashi Sado, National Institute of Genetics in 2006. R1 ES cells were used to generate the mutant mice. A loxP flanked neo cassette was removed by crossing with cre mice (B6).
Strain description Xist<IVS19> mice. A second intron of the human gamma-globin gene was inserted into exon 1 of Xist gene. Heterozygous mutant females with paternal mutant allele were embryonic lethality by non-random and abnormal X-inactivation. Tsix knockout (Xist<1loxGFP>)(RBRC01949), Tsix splising (Tsix<SA>)(RBRC01949), Xist/Tsix double knockout (X<dc>)(RBRC01950), Tsix<pA> (RBRC02653), Xist<IVS> (RBRC02654), Xist<delta> (RBRC02655).
Colony maintenance Heterozygote (female) x Wild-type (male) [C57BL/6JJcl]
Health Report
Gene Details
Promoter No Data
1 Symbol Xist
Symbol name inactive X specific transcripts
Chromosome X
Common name No Data
Symbol description No Data
2 Symbol SA
Symbol name Splice acceptor (human)
Chromosome X
Common name No Data
Symbol description No Data
3 Symbol Splice donor
Symbol name splicing donar (human)
Chromosome X
Common name No Data
Symbol description No Data
4 Symbol loxP
Symbol name phage P1 loxP
Chromosome X
Common name No Data
Symbol description No Data
References Development. 2008 Jan;135(2):227-35. Epub 2007 Dec 5.

Crucial role of antisense transcription across the Xist promoter in Tsix-mediated Xist chromatin modification.
Research applications Cre/loxP system,
Developmental Biology Research
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR.
The RECIPIENT of BIOLOGICAL RESOURCE must obtain a prior written consent on use of it from the DEPOSITOR/DEVELOPER.
Additional information
1 Mouse of the Month Feb 2009
2 Lab HP (Japanese)
3 Genotyping protocol <PCR>
Depositor Sado, Takashi (Medical institute of bioregulation, Kyushu University)
Strain Status /
Availability
(Expected delivery)


Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months