RBRC02657, B6.129P2-Gt(ROSA)26Sor<tm1(NLS-lacZ)Ito>

RBRC No. RBRC02657
Type Targeted MutationCartagena
Species Mus musculus
Strain name B6.129P2-Gt(ROSA)26Sor<tm1(NLS-lacZ)Ito>
Former Common name ROSA nls LacZ
H-2 Haplotype No Data
ES cell line E14 [129P2/OlaHsd]
Background strain No Data
Appearance
1 Appearance No Data
Genotype No Data
Strain development Developed by Shigeyoshi Itohara, Brain Science Institute, RIKEN. E14 ES cells derived from 129P2/OlaHsd were used. The mutant mice were backcrossed to C57BL/6J.
Strain description PGK-neo cassette flanked by loxP sites and NLS-lacZ were inserted into the Gt(ROSA)26Sor locus. The NLS-lacZ expression can be generated by crossing with tissue-specific Cre mice. The expression is localized within cell nucleus. Homozygous mutant mice show no obvious abnormality.
Colony maintenance Homozygote x Homozygote [or Crossing to C57BL/6JJcl]
Health Report
Gene Details
Promoter No Data
1 Symbol Gt(ROSA)26Sor
Symbol name gene trap ROSA 26, Philippe Soriano
Chromosome 6
Common name No Data
Symbol description No Data
2 Symbol lacZ
Symbol name beta-galactosidase (E. coli)
Chromosome 6
Common name No Data
Symbol description E. coli beta galactosidase
3 Symbol loxP
Symbol name phage P1 loxP
Chromosome 6
Common name No Data
Symbol description No Data
4 Symbol nls
Symbol name SV40 nuclear localaization signal (chemically-synthesized nucleotide)
Chromosome 6
Common name No Data
Symbol description No Data
Promoter mouse phosphoglycerate kinase promoter (PGK promoter)
5 Symbol neo
Symbol name neomycin resistance gene (E. coli)
Chromosome 6
Common name neo; neomycin;
Symbol description No Data
References

Research applications Cre/loxP system,
Fluorescent Proteins/lacZ System
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
Front. Behav. Neurosci., 7:17, (2013).
In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
Additional information
1 Mouse of the Month Mar 2013
2 Lab HP
3 Genotyping protocol <PCR>
Depositor Itohara, ShigeYoshi (RIKEN Center for Brain Science) Itohara, ShigeYoshi
Strain Status /
Availability
(Expected delivery)


Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months

Live
Live mouse :
BRC mice in Publications
Title Journal
(PMID)
Author
Novel reporter and deleter mouse strains generated using VCre/VloxP and SCre/SloxP systems, and their system specificity in mice. Transgenic Res. (2018).(29546522)
Yoshimura Y, Ida-Tanaka M, Hiramaki T, Goto M, Kamisako T, Eto T, Yagoto M, Kawai K, Takahashi T, Nakayama M, Ito M.