RBRC02704, B6.Cg-Tg(FucciS/G2/M)#474Bsi

RBRC No. RBRC02704
Type Transgene CongenicCartagena
Species Mus musculus
Strain name B6.Cg-Tg(FucciS/G2/M)#474Bsi
Former Common name B6.Cg-Tg(Fucci)474Bsi, B6;B6D2-Tg(Fucci)474Bsi, B6;B6D2-Tg(Fucci)474Bsi
H-2 Haplotype No Data
Background strain No Data
Appearance
1 Appearance black
Genotype a/a B/B C/C
Strain development Developed by Asako Sakaue-Sawano and Atsushi Miyawaki, RIKEN Brain Science Institute. The transgene was injected into the pronuclei of B6D2F1 fertilized eggs. The mice were backcrossed to C57BL/6N.
Strain description Transgenic mouse strains express fucci (fluorescent, ubiquitination-based cell cycle indicator) under the control of CAG promoter. These mice can be used to clearly visualize the cell cycle with fluorescence, i.e. S/G2/M phase nuclei green and those in G1 phase red in vivo. #474 (RBRC02704), #492 (RBRC02705), #504 (RBRC02706): Fucci-S/G2/M-Green (mAG-hGeminin(1/110)). #596 (RBRC02707), #610 (RBRC02708), #639 (RBRC02709): Fucci-G1-Red (mKO2-hCdt1(30/120)). For whole body (various tissues): #504 and #596 (Cell 2008). For hematopoietic system (monocyte and macrophage): #474 and #610 (Development 2013). For lymphocytic system: #492 and 639 (PNAS 2010). #474 and #610 double transgenic mice (RBRC02817). #504 and #596 double transgenic mice (RBRC02892). Whole-body visualization of Fucci mice with a flashlight (PCR genotyping is also available.): Check the Fucci S/2G/M-Green expressing mice at 1-4 days after birth by using blue LED flashlight through a sharp-cut filter in the dark box. Check the Fucci G1-Red expressing mice within 1-4 days after birth by using green LED flashlight through a green band-cut filter in the dark box. The part with few hairs such as in the mouth and auricles is suitable for the observation of fluorescence. Visualizing device: blue LED flashlight (470 nm), S470/30 filter, 535AF26 filter, sharp-cut filter (500 or 520 nm), green band-cut filter (red filter). #474 and #504: Tg homozygous mice are viable and fertile. #596: Tg homozygous mice are probably lethal (details unknown). #492, #610 and #639: Viability and fertility of homozygous mice are unknown (probably decreased fertility, details unknown).
Colony maintenance Carrier x Carrier [or Crossing to C57BL/6NJcl]
Health Report
Gene Details
Promoter No Data
1 Symbol GMNN
Symbol name geminin (human)
Chromosome UN
Common name No Data
Symbol description No Data
Promoter CAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA)
2 Symbol mAG
Symbol name monomeric Azami Green (Coral)
Chromosome UN
Common name No Data
Symbol description No Data
References Cell. 2008 Feb 8;132(3):487-98.

Visualizing spatiotemporal dynamics of multicellular cell-cycle progression.
Visualizing spatiotemporal dynamics of multicellular cell-cycle progression.
Visualizing spatiotemporal dynamics of multicellular cell-cycle progression.
Research applications Fluorescent Proteins/lacZ System
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
PLOS ONE (2013) doi: 10.1371/journal.pone.0073801. Development (2013) doi:10.1242/dev.099226.
The RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial. The RECIPIENT agrees to acknowledge PROVIDER in all oral presentations and written publications as related to the MATERIAL and provide a copy of the publication to the DEPOSITOR. The RECIPIENT of the BIOLOGICAL RESOURCE is requested to make a Material Transfer Agreement regarding the usage of the CAG promoter specified by the DEVELOPER, Dr. Junichi Miyazaki of Osaka University.
Additional information
1 Genetic Background
2 CAGGS MATERIAL TRANSFER AGREEMENT (Pdf)/ (Word)
Miyawaki Lab HP
3 Genotyping protocol <PCR>
Depositor Miyawaki, Atsushi (RIKEN Center for Brain Science) Miyawaki, Atsushi
Strain Status /
Availability
(Expected delivery)


Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months

Live
Live mouse :
BRC mice in Publications
Title Journal
(PMID)
Author
Tracking of intertissue migration reveals the origins of tumor-infiltrating monocytes. Proc Natl Acad Sci U S A. (2014).(24825888)
Shand FH, Ueha S, Otsuji M, Koid SS, Shichino S, Tsukui T, Kosugi-Kanaya M, Abe J, Tomura M, Ziogas J, Matsushima K.
Fucci-guided purification of hematopoietic stem cells with high repopulating activity. Biochem Biophys Res Commun. (2014).(25534850)
Yo M, Sakaue-Sawano A, Noda S, Miyawaki A, Miyoshi H.
Involvement of receptor activator of nuclear factor kappa-B ligand-induced incomplete cytokinesis in polyploidization of osteoclasts. J. Biol. Chem. (2015).(26670608)
Takegahara N, Kim H, Mizuno H, Sakaue-Sawano A, Miyawaki A, Tomura M, Kanagawa O, Ishii M, Choi Y.
Microbiome Influences Prenatal and Adult Microglia in a Sex-Specific Manner. Cell172(3): 500-516 (2018).(29275859)
Morgane Sonia Thion, Donovan Low, Aymeric Silvin, Jinmiao Chen, Pauline Grisel, Jonas Schulte-Schrepping, Ronnie Blecher, Thomas Ulas, Paola Squarzoni, Guillaume Hoeffel, Fanny Coulpier, Eleni Siopi, Friederike Sophie David, Claus Scholz, Foo Shihui, Josephine Lum, Arlaine Anne Amoyo, Anis Larbi, Michael Poidinger, Anne Buttgereit, Pierre-Marie Lledo, Melanie Greter, Jerry Kok Yen Chan, Ido Amit, Marc Beyer, Joachim Ludwig Schultze, Andreas Schlitzer, Sven Pettersson, Florent Ginhoux, Sonia Garel.
Developmental Analysis of Bone Marrow Neutrophils Reveals Populations Specialized in Expansion, Trafficking, and Effector Functions. Immunity (2018).(29466759)
Evrard M, Kwok IWH, Chong SZ, Teng KWW, Becht E, Chen J, Sieow JL, Penny HL, Ching GC, Devi S, Adrover JM, Li JLY, Liong KH, Tan L, Poon Z, Foo S, Chua JW, Su IH, Balabanian K, Bachelerie F, Biswas SK, Larbi A, Hwang WYK, Madan V, Koeffler HP, Wong SC, Newell EW, Hidalgo A, Ginhoux F, Ng LG.