RBRC02707, B6.Cg-Tg(FucciG1)#596Bsi

RBRC No. RBRC02707
Type Transgene CongenicCartagena
Species Mus musculus
Strain name B6.Cg-Tg(FucciG1)#596Bsi
Former Common name B6.Cg-Tg(Fucci)596Bsi, Fucci G1-red#596, B6;B6D2-Tg(Fucci)596Bsi
H-2 Haplotype No Data
Background strain C57BL/6N and DBA/2N mixed
Appearance
1 Appearance black
Genotype a/a B/B C/C
Strain development Developed by Asako Sakaue-Sawano and Atsushi Miyawaki, RIKEN Brain Science Institute. The transgene was injected into the pronuclei of B6D2F1 fertilized eggs. The mice were backcrossed to C57BL/6N.
Strain description Transgenic mouse strains express fucci (fluorescent, ubiquitination-based cell cycle indicator) under the control of CAG promoter. These mice can be used to clearly visualize the cell cycle with fluorescence, i.e. S/G2/M phase nuclei green and those in G1 phase red in vivo. #474 (RBRC02704), #492 (RBRC02705), #504 (RBRC02706): Fucci-S/G2/M-Green (mAG-hGeminin(1/110)). #596 (RBRC02707), #610 (RBRC02708), #639 (RBRC02709): Fucci-G1-Red (mKO2-hCdt1(30/120)). For whole body (various tissues): #504 and #596 (Cell 2008). For hematopoietic system (monocyte and macrophage): #474 and #610 (Development 2013). For lymphocytic system: #492 and 639 (PNAS 2010). #474 and #610 double transgenic mice (RBRC02817). #504 and #596 double transgenic mice (RBRC02892). Whole-body visualization of Fucci mice with a flashlight (PCR genotyping is also available.): Check the Fucci S/2G/M-Green expressing mice at 1-4 days after birth by using blue LED flashlight through a sharp-cut filter in the dark box. Check the Fucci G1-Red expressing mice within 1-4 days after birth by using green LED flashlight through a green band-cut filter in the dark box. The part with few hairs such as in the mouth and auricles is suitable for the observation of fluorescence. Visualizing device: blue LED flashlight (470 nm), S470/30 filter, 535AF26 filter, sharp-cut filter (500 or 520 nm), green band-cut filter (red filter). #474 and #504: Tg homozygous mice are viable and fertile. #596: Tg homozygous mice are probably lethal (details unknown). #492, #610 and #639: Viability and fertility of homozygous mice are unknown (probably decreased fertility, details unknown).
Colony maintenance Carrier x Noncarrier [C57BL/6NJcl]
Health Report
Gene Details
Promoter No Data
1 Symbol CDT1
Symbol name chromatin licensing and DNA replication factor (human)
Chromosome UN
Common name No Data
Symbol description No Data
Promoter CAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA)
2 Symbol mKO2
Symbol name monomeric Kusabira Orange (Coral)
Chromosome UN
Common name No Data
Symbol description No Data
References Cell. 2008 Feb 8;132(3):487-98.

Visualizing spatiotemporal dynamics of multicellular cell-cycle progression.
Visualizing spatiotemporal dynamics of multicellular cell-cycle progression.
Research applications Fluorescent Proteins/lacZ System
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
Cell, 132, 487-498 (2008). Gene, 108, 193-200 (1991).
Distribution of the BIOLOGICAL RESOURCE is limited to for academic researches in not-for-profit entities. Any use of the BIOLOGICAL RESOURCE for profit purposes by the RECIPIENT, such as any use by for-profit entities, any use for the BIOLOGICAL RESOURCE by not-for-profit entities in researches funded in any part by or in collaboration with for-profit entities, or any use the BIOLOGICAL RESOURCE by the not-for-profit entities for patent filing or licensing, requires a separate license from MBL, prior to distribution.
Additional information
1 Mouse of the Month Mar 2009
Genetic Background
Breeding characters
2 Miyawaki Lab HP
3 Genotyping protocol <PCR>
Depositor MEDICAL & BIOLOGICAL LABORATORIES CO., LTD., (Medical Biological Laboratories Co.,Ltd) MEDICAL & BIOLOGICAL LABORATORIES CO., LTD.,
Strain Status /
Availability
(Expected delivery)


Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months

Live
Live mouse :
BRC mice in Publications
Title Journal
(PMID)
Author
Functional tooth restoration utilising split germs through re-regionalisation of the tooth-forming field. Sci. Rep.5: 18393 (2015).(26673152)
Yamamoto N, Oshima M, Tanaka C, Ogawa M, Nakajima K, Ishida K, Moriyama K, Tsuji T.
Coordination of Cellular Dynamics Contributes to Tooth Epithelium Deformations. PLoS One. (2016).(27588418)
Morita R, Kihira M, Nakatsu Y, Nomoto Y, Ogawa M, Ohashi K, Mizuno K, Tachikawa T, Ishimoto Y, Morishita Y, Tsuji T.