RBRC02708, B6.Cg-Tg(FucciG1)#610Bsi

RBRC No. RBRC02708
Type Transgene CongenicCartagena
Species Mus musculus
Strain name B6.Cg-Tg(FucciG1)#610Bsi
Former Common name B6.Cg-Tg(Fucci)610Bsi, B6;B6D2-Tg(Fucci)610Bsi
H-2 Haplotype No Data
Background strain No Data
Appearance
1 Appearance No Data
Genotype No Data
Strain development Developed by Asako Sakaue-Sawano and Atsushi Miyawaki, RIKEN Brain Science Institute. The transgene was injected into the pronuclei of B6D2F1 fertilized eggs. The mice were backcrossed to C57BL/6N.
Strain description Transgenic mouse strains express fucci (fluorescent, ubiquitination-based cell cycle indicator) under the control of CAG promoter. These mice can be used to clearly visualize the cell cycle with fluorescence, i.e. S/G2/M phase nuclei green and those in G1 phase red in vivo. #474 (RBRC02704), #492 (RBRC02705), #504 (RBRC02706): Fucci-S/G2/M-Green (mAG-hGeminin(1/110)). #596 (RBRC02707), #610 (RBRC02708), #639 (RBRC02709): Fucci-G1-Red (mKO2-hCdt1(30/120)). For whole body (various tissues): #504 and #596 (Cell 2008). For hematopoietic system (monocyte and macrophage): #474 and #610 (Development 2013). For lymphocytic system: #492 and 639 (PNAS 2010). #474 and #610 double transgenic mice (RBRC02817). #504 and #596 double transgenic mice (RBRC02892). Whole-body visualization of Fucci mice with a flashlight (PCR genotyping is also available.): Check the Fucci S/2G/M-Green expressing mice at 1-4 days after birth by using blue LED flashlight through a sharp-cut filter in the dark box. Check the Fucci G1-Red expressing mice within 1-4 days after birth by using green LED flashlight through a green band-cut filter in the dark box. The part with few hairs such as in the mouth and auricles is suitable for the observation of fluorescence. Visualizing device: blue LED flashlight (470 nm), S470/30 filter, 535AF26 filter, sharp-cut filter (500 or 520 nm), green band-cut filter (red filter). #474 and #504: Tg homozygous mice are viable and fertile. #596: Tg homozygous mice are probably lethal (details unknown). #492, #610 and #639: Viability and fertility of homozygous mice are unknown (probably decreased fertility, details unknown).
Colony maintenance Carrier x Noncarrier [C57BL/6NJcl]
Health Report
Gene Details
Promoter No Data
1 Symbol CDT1
Symbol name chromatin licensing and DNA replication factor (human)
Chromosome UN
Common name No Data
Symbol description No Data
Promoter CAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA)
2 Symbol mKO2
Symbol name monomeric Kusabira Orange (Coral)
Chromosome UN
Common name No Data
Symbol description No Data
References Cell. 2008 Feb 8;132(3):487-98.

Visualizing spatiotemporal dynamics of multicellular cell-cycle progression.
Visualizing spatiotemporal dynamics of multicellular cell-cycle progression.
Research applications Fluorescent Proteins/lacZ System
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
Development (2013) doi:10.1242/dev.099226.
The RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial. The RECIPIENT agrees to acknowledge PROVIDER in all oral presentations and written publications as related to the MATERIAL and provide a copy of the publication to the DEPOSITOR.
Additional information
1 Genetic Background
2 CAGGS MATERIAL TRANSFER AGREEMENT (Pdf)/ (Word)
Miyawaki Lab HP
3 Genotyping protocol <PCR>
Depositor Miyawaki, Atsushi (RIKEN Center for Brain Science) Miyawaki, Atsushi
Strain Status /
Availability
(Expected delivery)


Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months

Live
Live mouse :
BRC mice in Publications
Title Journal
(PMID)
Author
Fucci-guided purification of hematopoietic stem cells with high repopulating activity. Biochem Biophys Res Commun. (2014).(25534850)
Yo M, Sakaue-Sawano A, Noda S, Miyawaki A, Miyoshi H.