RBRC02716, B6.129X1-Plce1<tm1Tkat>

RBRC No. RBRC02716
Type Targeted MutationCartagena wks
Species Mus musculus
Strain name B6.129X1-Plce1<tm1Tkat>
Former Common name PLCεKO
H-2 Haplotype No Data
Background strain No Data
1 Appearance black
Genotype a/a B/B C/C
Strain development Developed by Drs. T. Kataoka, N. Suzuki and H. Saito in 2001.
Strain description Phospholipase Cepsilon is a novel class of phosphoinositide-specific phospholipase C that were identified as a downstream effector of Ras and Rap small GTPases. This knockout strain expesses a phospolipase Cepsilon catalytically inactivated by gene targeting. Homozygous mutant mice show a congenital semilunar valvulogenesis defect.
Colony maintenance Backcross to C57BL/6 (Heterozygote x C57BL/6JJcl)
Health Report
Gene Details
Promoter No Data
1 Symbol Plce1
Symbol name phospholipase C, epsilon 1
Chromosome 19
Common name 4933403A21Rik, PLCepsilon
Symbol description No Data
Promoter mouse phosphoglycerate kinase promoter (PGK promoter)
2 Symbol neo
Symbol name neomycin resistance gene (E. coli)
Chromosome 19
Common name neo; neomycin;
Symbol description No Data

Research applications Cardiovascular Research,
Mouse Models for Human Disease
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
Mol. Cell Biol., 25, 2191-2199 (2005). Cancer Res., 64, 8808-8810 (2004).
In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for publication of research papers. The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use with the results from the use of the BIOLOGICAL RESOURCE.
Additional information
1 Lab HP (Japanese)
2 Genotyping protocol <PCR>
Depositor Kataoka, Tohru (Kobe University) Kataoka, Tohru
Strain Status /
(Expected delivery)

Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months