RBRC02794, B6.Cg-Vps52<tm1Kab>/95Rbrc

RBRC No. RBRC02794
Type Targeted MutationCartagena
Species Mus musculus
Strain name B6.Cg-Vps52<tm1Kab>/95Rbrc
Former Common name Vps52 #95(Acc.No.CDB0767K)
H-2 Haplotype No Data
ES cell line TT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1]
Background strain No Data
Appearance
1 Appearance No Data
Genotype No Data
Strain development Developed by Michihiko Sugimoto and Kuniya Abe, RIKEN BioResource center in 2008. TT2 (B6CBF1 derived) ES cells were used to generate the targeted mice. C57BL/6 and CBA mixed (C57BL/6 semicongenic).
Strain description Vps52 2lox2frt (neo) targeted mice in which Frt-neo cassette (PGK-neo) was inserted upstream of exon 2, and two loxP sites were located in introns 1 and 2. Homozygous mutant mice were embryonic lethal. Line 95 (main line) (RBRC02794), Line 16 (sub line) (RBRC02832), Line 20 (sub line) (RBRC02857).
Colony maintenance Heterozygote x Wild-type [C57BL/6NCrlCrlj]
Health Report
Gene Details
Promoter No Data
1 Symbol Vps52
Symbol name VPS52 GARP complex subunit
Chromosome 17
Common name No Data
Symbol description No Data
2 Symbol Frt
Symbol name yeast FRT (flippase recombination target) site
Chromosome 17
Common name No Data
Symbol description No Data
3 Symbol Frt
Symbol name yeast FRT (flippase recombination target) site
Chromosome 17
Common name No Data
Symbol description No Data
4 Symbol HBB2
Symbol name rabbit beta-globin
Chromosome 17
Common name No Data
Symbol description No Data
5 Symbol lox71
Symbol name mutant lox sequence
Chromosome 17
Common name No Data
Symbol description No Data
6 Symbol loxP
Symbol name phage P1 loxP
Chromosome 17
Common name No Data
Symbol description No Data
Promoter mouse phosphoglycerate kinase promoter (PGK promoter)
7 Symbol neo
Symbol name neomycin resistance gene (E. coli)
Chromosome 17
Common name neo; neomycin;
Symbol description No Data
References

Research applications Cre/loxP system,
Developmental Biology Research,
FLP/frt system
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
(1) The RECIPIENT shall obtain a prior agreement from Dr. Kuniya Abe (abe@brc.riken.jp) to transfer the BIOLOGICAL RESOURCE. (2) In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the Acc.No. CDB0767K and Web. Site (http://www.cdb.riken.jp/arg/mutant%20mice%20list.html) is requested. (3) In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the literature designed by the DEPOSITOR is requested (Cell Rep. 2012 2(5):1363-74.). (4) Please inform the DEPOSITOR (mutant@cdb.riken.jp) about the paper when published.
Additional information
1 Lab HP
2 Genotyping protocol <PCR>
Depositor RIKEN, CDB (RIKEN Center for Developmental Biology) RIKEN, CDB
Strain Status /
Availability
(Expected delivery)


Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months