RBRC02831, B6.129S6-Gcg<tm1Yhys>

RBRC No. RBRC02831
Type Targeted MutationCartagena wks
Species Mus musculus
Strain name B6.129S6-Gcg<tm1Yhys>
Former Common name No Data
H-2 Haplotype No Data
Background strain C57BL/6JJmsSlc
Appearance
1 Appearance black
Genotype a/a B/B C/C
Strain development Developed by Dr. Yoshitaka Hayashi, Nagoya University in 2007. Gcg gene was disrupted by introduction of GFP cDNA. GFP expresses in Gcg positive cells.
Strain description Glucagon, Gcg deficient mice. Homozygous mutant mice lack peptides derived from proglucagon.
Colony maintenance Heterozygote x Wild-type [C57BL/6JJmsSlc]
Health Report
Gene Details
Promoter No Data
1 Symbol Gcg
Symbol name glucagon
Chromosome 2
Common name No Data
Symbol description No Data
2 Symbol GFP
Symbol name Green Fluorescent Protein (Jellyfish)
Chromosome No Data
Common name No Data
Symbol description No Data
Promoter mouse phosphoglycerate kinase promoter (PGK promoter)
3 Symbol neo
Symbol name neomycin resistance gene (E. coli)
Chromosome No Data
Common name neo; neomycin;
Symbol description No Data
References

Research applications Fluorescent Proteins/lacZ System
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR.
The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for publication of research papers. The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use with the results from the use of the BIOLOGICAL RESOURCE.
Additional information
1 Mouse of the Month Nov 2012
2 GFP Transfer License (Japanese / English)
Please fill in the Schedule A, and submit two signed copies to us together with two signed copies of RIKEN BRC’s MTA. Please also read Schedule B.
3 Genotyping protocol <PCR>
Depositor Hayashi, Yoshitaka (Nagoya University) Hayashi, Yoshitaka
Strain Status /
Availability
(Expected delivery)


Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months
BRC mice in Publications
Title Journal
(PMID)
Author
Ectopic Expression of GIP in Pancreatic beta-Cells Maintains Enhanced Insulin Secretion in Mice With Complete Absence of Proglucagon-Derived Peptides. Diabetes.62(2): 510-8 (2013).(23099862)
Fukami A, Seino Y, Ozaki N, Yamamoto M, Sugiyama C, Sakamoto-Miura E, Himeno T, Takagishi Y, Tsunekawa S, Ali S, Drucker DJ, Murata Y, Seino Y, Oiso Y, Hayashi Y.