RBRC02975, B6.129S-Atg5<tm1Myok>

RBRC No. RBRC02975
Type Targeted MutationCartagena wks
Species Mus musculus
Strain name B6.129S-Atg5<tm1Myok>
Former Common name Atg5flox
H-2 Haplotype No Data
ES cell line CCE/EK.CCE [129S/SvEv-Gpi1]
Background strain C57BL/6
Appearance
1 Appearance black
Genotype a/a B/B C/C
Strain development Developed by Kenji Nakamura, Mitsubishi Kagaku Institute of Life Sciences and Noboru Mizushima, Tokyo Metropolitan Institute of Medical Science in 2003. The construct was electoroporated into CCE/EK.CCE ES cells derived from 129S/SvEv-Gpi1<c>. The mutant mice were backcrossed to C57BL/6J.
Strain description Atg5 floxed mice. Exon 3 of Atg5 gene was flanked by loxP sites containing neomycin resistant cassette. Autophagy is an intracellular degradation process by an autophagosome, which contains a portion of cytoplasm and subsequently degrades upon fusion with a lysosome. Autophagy is considered to be important for the cellular response to starvation and the normal turnover of cytoplasmic components as well. Atg5, autophagy-related 5 is essential for autophagosome formation. Conditional B6.129S-Atg5<tm1Myok> (RBRC02975) mice can be crossed with various tissue-specific Cre transgenic mice to study the role of autophagy in adult tissues. Null knockout mice are also available: B6;129-Atg5<tm1Nmz> (RBRC02231).
Colony maintenance Homozygote x Homozygote [or Crossing to C57BL/6JJmsSlc]
Health Report
Gene Details
Promoter No Data
1 Symbol Atg5
Symbol name autophagy-related 5 (yeast)
Chromosome 10
Common name No Data
Symbol description No Data
2 Symbol loxP
Symbol name phage P1 loxP
Chromosome 10
Common name No Data
Symbol description No Data
Promoter herpes simplex virus thymidine kinase promoter (HSV tk promoter)
3 Symbol neo
Symbol name neomycin resistance gene (E. coli)
Chromosome 10
Common name neo; neomycin;
Symbol description No Data
References Nature. 2006 Jun 15;441(7095):885-9. Epub 2006 Apr 19.

Suppression of basal autophagy in neural cells causes neurodegenerative disease in mice.
Research applications Cell Biology Research,
Cre/loxP system
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
Nature, 441, 885-889 (2006).
In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
BIOLOGICAL RESOURCE is limited to for academic research in non-profit organization. Any use of the BIOLOGICAL RESOURCE for profit purposes by a non-profit organization or any use of the BIOLOGICAL RESOURCE by a profit organization requires a separate license from the DEPOSITOR prior to distribution. The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
Additional information
1 Mouse of the Month Jul 2009
Genetic Background
Breeding characters
2 Lab HP
3 Genotyping protocol <PCR>
Depositor Mizushima, Noboru (The University of Tokyo, Graduate School of Medicine) Mizushima, Noboru
Strain Status /
Availability
(Expected delivery)


Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months

Live
Live mouse :
BRC mice in Publications
Title Journal
(PMID)
Author
Liver-specific loss of Atg5 causes persistent activation of Nrf2 and protects against acetaminophen-induced liver injury. Toxicol Sci.127(2): 438-50 (2012).(22491424)
Ni HM, Boggess N, McGill MR, Lebofsky M, Borude P, Apte U, Jaeschke H, Ding WX.
Autophagy regulates endothelial cell processing, maturation and secretion of von Willebrand factor. Nat Med.19(10): 1281-7 (2013).(24056772)
Torisu T, Torisu K, Lee IH, Liu J, Malide D, Combs CA, Wu XS, Rovira II, Fergusson MM, Weigert R, Connelly PS, Daniels MP, Komatsu M, Cao L, Finkel T.
p53 status determines the role of autophagy in pancreatic tumour development. Nature. (2013).(24305049)
Rosenfeldt MT, O’Prey J, Morton JP, Nixon C, Mackay G, Mrowinska A, Au A, Rai TS, Zheng L, Ridgway R, Adams PD, Anderson KI, Gottlieb E, Sansom OJ, Ryan KM.
Induction of autophagy supports the bioenergetic demands of quiescent muscle stem cell activation. EMBO J. (2014).(25316028)
Tang AH, Rando TA.
Aspergillus Cell Wall Melanin Blocks LC3-Associated Phagocytosis to Promote Pathogenicity. Cell Host Microbe.19(1): 79-90 (2015).(26749442)
Akoumianaki T, Kyrmizi I, Valsecchi I, Gresnigt MS, Samonis G, Drakos E, Boumpas D, Muszkieta L, Prevost MC, Kontoyiannis DP, Chavakis T, Netea MG, van de Veerdonk FL, Brakhage AA, El-Benna J, Beauvais A, Latge JP, Chamilos G.
Autophagy deficiency in macrophages enhances NLRP3 inflammasome activity and chronic lung disease following silica exposure. Toxicol. Appl. Pharmacol. (2016).(27594529)
Jessop F, Hamilton RF, Rhoderick JF, Shaw PK, Holian A.
Cardioprotection and lifespan extension by the natural polyamine spermidine. Nat. Med.22(12): 1428-1438 (2016).(27841876)
Eisenberg T, Abdellatif M, Schroeder S, Primessnig U, Stekovic S, Pendl T, Harger A, Schipke J, Zimmermann A, Schmidt A, Tong M, Ruckenstuhl C, Dammbrueck C, Gross AS, Herbst V, Magnes C, Trausinger G, Narath S, Meinitzer A, Hu Z, Kirsch A, Eller K, Carmona-Gutierrez D, Büttner S, Pietrocola F, Knittelfelder O, Schrepfer E, Rockenfeller P, Simonini C, Rahn A, Horsch M, Moreth K, Beckers J, Fuchs H, Gailus-Durner V, Neff F, Janik D, Rathkolb B, Rozman J, de Angelis MH, Moustafa T, Haemmerle G, Mayr M, Willeit P, von Frieling-Salewsky M, Pieske B, Scorrano L, Pieber T, Pechlaner R, Willeit J, Sigrist SJ, Linke WA, Mühlfeld C, Sadoshima J, Dengjel J, Kiechl S, Kroemer G, Sedej S, Madeo F.
Early AMD-like defects in the RPE and retinal degeneration in aged mice with RPE-specific deletion of Atg5 or Atg7. Mol. Vis.23: 228-241 (2017).(28465655)
Zhang Y, Cross SD, Stanton JB, Marmorstein AD, Le YZ, Marmorstein LY.
Dietary PUFAs attenuate NLRP3 inflammasome activation via enhancing macrophage autophagy. J. Lipid Res.58(9): 1808-1821 (2017).(28729463)
Shen L, Yang Y, Ou T, Key CC, Tong SH, Sequeira RC, Nelson JM, Nie Y, Wang Z, Boudyguina E, Shewale SV, Zhu X.
Knockout of autophagy gene, ATG5 in mice vaginal cells abrogates cytokine response and pathogen clearance during vaginal infection of Candida albicans. Cell Immunol. (2017).(29306553)
Shroff A, Sequeira R, Patel V, Reddy KVR.
Autophagy orchestrates the regulatory program of tumor-associated myeloid-derived suppressor cells. J. Clin. Invest. (2018).(29920188)
Alissafi T, Hatzioannou A, Mintzas K, Barouni RM, Banos A, Sormendi S, Polyzos A, Xilouri M, Wielockx B, Gogas H, Verginis P.
Differential and convergent utilization of autophagy components by positive-strand RNA viruses. PLoS Biol. (2019).(30608919)
Abernathy E, Mateo R, Majzoub K, van Buuren N, Bird SW, Carette JE, Kirkegaard K.